Detection of Low Molecular Weight IReduced Zein Polypeptides Separated by Polyacrylamide Gel Electrophoresis

SHELA GORINSTEIN*, GEORGE V. QUICKE, ANNE M. PHILLIPS

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Several bands including some low molecular weight (MW) peptides [9 and 11 kd (Kilo‐Dalton)] were visualized in reduced zeins after separation by sodium dodecyl sulphate polyacrylamide gel electro‐phoresis (SDS‐PAGE). It was concluded that 40% isopropanol‐10% acetic acid was effective in fixing these fast moving polypeptides. The demonstration of higher MW (40‐45 Kd) polypeptides may have also resulted from this treatment.

Original languageEnglish
Pages (from-to)1366-1367
Number of pages2
JournalJournal of Food Science
Volume51
Issue number5
DOIs
StatePublished - Sep 1986

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