Detection of Low Molecular Weight IReduced Zein Polypeptides Separated by Polyacrylamide Gel Electrophoresis

SHELA GORINSTEIN; GEORGE V. QUICKE; ANNE M. PHILLIPS

doi:10.1111/j.1365-2621.1986.tb13125.x

Detection of Low Molecular Weight IReduced Zein Polypeptides Separated by Polyacrylamide Gel Electrophoresis

SHELA GORINSTEIN^*
, GEORGE V. QUICKE
, ANNE M. PHILLIPS

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Several bands including some low molecular weight (MW) peptides [9 and 11 kd (Kilo‐Dalton)] were visualized in reduced zeins after separation by sodium dodecyl sulphate polyacrylamide gel electro‐phoresis (SDS‐PAGE). It was concluded that 40% isopropanol‐10% acetic acid was effective in fixing these fast moving polypeptides. The demonstration of higher MW (40‐45 Kd) polypeptides may have also resulted from this treatment.

Original language	English
Pages (from-to)	1366-1367
Number of pages	2
Journal	Journal of Food Science
Volume	51
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2621.1986.tb13125.x
State	Published - Sep 1986

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title = "Detection of Low Molecular Weight IReduced Zein Polypeptides Separated by Polyacrylamide Gel Electrophoresis",

abstract = "Several bands including some low molecular weight (MW) peptides [9 and 11 kd (Kilo‐Dalton)] were visualized in reduced zeins after separation by sodium dodecyl sulphate polyacrylamide gel electro‐phoresis (SDS‐PAGE). It was concluded that 40\% isopropanol‐10\% acetic acid was effective in fixing these fast moving polypeptides. The demonstration of higher MW (40‐45 Kd) polypeptides may have also resulted from this treatment.",

author = "SHELA GORINSTEIN and QUICKE, \{GEORGE V.\} and PHILLIPS, \{ANNE M.\}",

year = "1986",

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doi = "10.1111/j.1365-2621.1986.tb13125.x",

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AU - GORINSTEIN, SHELA

AU - QUICKE, GEORGE V.

AU - PHILLIPS, ANNE M.

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Y1 - 1986/9

N2 - Several bands including some low molecular weight (MW) peptides [9 and 11 kd (Kilo‐Dalton)] were visualized in reduced zeins after separation by sodium dodecyl sulphate polyacrylamide gel electro‐phoresis (SDS‐PAGE). It was concluded that 40% isopropanol‐10% acetic acid was effective in fixing these fast moving polypeptides. The demonstration of higher MW (40‐45 Kd) polypeptides may have also resulted from this treatment.

AB - Several bands including some low molecular weight (MW) peptides [9 and 11 kd (Kilo‐Dalton)] were visualized in reduced zeins after separation by sodium dodecyl sulphate polyacrylamide gel electro‐phoresis (SDS‐PAGE). It was concluded that 40% isopropanol‐10% acetic acid was effective in fixing these fast moving polypeptides. The demonstration of higher MW (40‐45 Kd) polypeptides may have also resulted from this treatment.

UR - https://www.scopus.com/pages/publications/84861654160

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ER -

Detection of Low Molecular Weight IReduced Zein Polypeptides Separated by Polyacrylamide Gel Electrophoresis

Abstract

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