Detection of Plasmodium falciparum in blood using DNA hybridization

Y. Pollack; S. Metzger; R. Shemer; D. Landau; D. T. Spira; J. Golenser

doi:10.4269/ajtmh.1985.34.663

Detection of Plasmodium falciparum in blood using DNA hybridization

Y. Pollack, S. Metzger, R. Shemer, D. Landau, D. T. Spira, J. Golenser

Department of Developmental Biology and Cancer Research

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A rapid and simple assay for detecting Plasmodium falciparum in human blood was developed. The assay is based on DNA-DNA spot hybridization, using radiolabeled P. falciparum DNA as a probe and finger prick blood as the assay sample. It is very sensitive, able to detect parasitemia levels of 0.0001% in 10 μl of blood. The assay can be quantified and used to estimate parasitemia levels. Several hundred blood samples can be processed simultaneously, and the entire procedure is completed within 24 hr. This assay can be useful for epidemiological surveys, for screening of blood by blood banks and for health authorities examining immigrants and tourists coming from malaria infested areas.

Original language	American English
Pages (from-to)	663-667
Number of pages	5
Journal	American Journal of Tropical Medicine and Hygiene
Volume	34
Issue number	4
DOIs	https://doi.org/10.4269/ajtmh.1985.34.663
State	Published - 1985

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10.4269/ajtmh.1985.34.663

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abstract = "A rapid and simple assay for detecting Plasmodium falciparum in human blood was developed. The assay is based on DNA-DNA spot hybridization, using radiolabeled P. falciparum DNA as a probe and finger prick blood as the assay sample. It is very sensitive, able to detect parasitemia levels of 0.0001% in 10 μl of blood. The assay can be quantified and used to estimate parasitemia levels. Several hundred blood samples can be processed simultaneously, and the entire procedure is completed within 24 hr. This assay can be useful for epidemiological surveys, for screening of blood by blood banks and for health authorities examining immigrants and tourists coming from malaria infested areas.",

author = "Y. Pollack and S. Metzger and R. Shemer and D. Landau and Spira, {D. T.} and J. Golenser",

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AU - Pollack, Y.

AU - Metzger, S.

AU - Shemer, R.

AU - Landau, D.

AU - Spira, D. T.

AU - Golenser, J.

PY - 1985

Y1 - 1985

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AB - A rapid and simple assay for detecting Plasmodium falciparum in human blood was developed. The assay is based on DNA-DNA spot hybridization, using radiolabeled P. falciparum DNA as a probe and finger prick blood as the assay sample. It is very sensitive, able to detect parasitemia levels of 0.0001% in 10 μl of blood. The assay can be quantified and used to estimate parasitemia levels. Several hundred blood samples can be processed simultaneously, and the entire procedure is completed within 24 hr. This assay can be useful for epidemiological surveys, for screening of blood by blood banks and for health authorities examining immigrants and tourists coming from malaria infested areas.

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JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 4

ER -

Detection of Plasmodium falciparum in blood using DNA hybridization

Abstract

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