TY - JOUR
T1 - Detection of Plasmodium falciparum in blood using DNA hybridization
AU - Pollack, Y.
AU - Metzger, S.
AU - Shemer, R.
AU - Landau, D.
AU - Spira, D. T.
AU - Golenser, J.
PY - 1985
Y1 - 1985
N2 - A rapid and simple assay for detecting Plasmodium falciparum in human blood was developed. The assay is based on DNA-DNA spot hybridization, using radiolabeled P. falciparum DNA as a probe and finger prick blood as the assay sample. It is very sensitive, able to detect parasitemia levels of 0.0001% in 10 μl of blood. The assay can be quantified and used to estimate parasitemia levels. Several hundred blood samples can be processed simultaneously, and the entire procedure is completed within 24 hr. This assay can be useful for epidemiological surveys, for screening of blood by blood banks and for health authorities examining immigrants and tourists coming from malaria infested areas.
AB - A rapid and simple assay for detecting Plasmodium falciparum in human blood was developed. The assay is based on DNA-DNA spot hybridization, using radiolabeled P. falciparum DNA as a probe and finger prick blood as the assay sample. It is very sensitive, able to detect parasitemia levels of 0.0001% in 10 μl of blood. The assay can be quantified and used to estimate parasitemia levels. Several hundred blood samples can be processed simultaneously, and the entire procedure is completed within 24 hr. This assay can be useful for epidemiological surveys, for screening of blood by blood banks and for health authorities examining immigrants and tourists coming from malaria infested areas.
UR - http://www.scopus.com/inward/record.url?scp=0022414074&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.1985.34.663
DO - 10.4269/ajtmh.1985.34.663
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AN - SCOPUS:0022414074
SN - 0002-9637
VL - 34
SP - 663
EP - 667
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 4
ER -
