TY - JOUR
T1 - Determination, by fluorescein diacetate staining, of fungal viability during mycoparasitism
AU - Barak, Rina
AU - Chet, Ilan
PY - 1986
Y1 - 1986
N2 - Trichoderma hamatum is a mycoparasite of Rhizoctonia solani. When stained with the vital stain fluorescein diacetate (FDA), both interacting fungi showed fluorescence during the first 24 h of incubation. Thereafter R. solani showed only a faint fluorescence while T. hamatum was still glowing up to 7 days. When each of these fungi were grown alone they showed a significant fluorescence for 4 days. This technique for determination of fungal viability during mycoparasitism may indicate that the initial attack occurs while the host is still alive.
AB - Trichoderma hamatum is a mycoparasite of Rhizoctonia solani. When stained with the vital stain fluorescein diacetate (FDA), both interacting fungi showed fluorescence during the first 24 h of incubation. Thereafter R. solani showed only a faint fluorescence while T. hamatum was still glowing up to 7 days. When each of these fungi were grown alone they showed a significant fluorescence for 4 days. This technique for determination of fungal viability during mycoparasitism may indicate that the initial attack occurs while the host is still alive.
UR - http://www.scopus.com/inward/record.url?scp=38249041941&partnerID=8YFLogxK
U2 - 10.1016/0038-0717(86)90067-2
DO - 10.1016/0038-0717(86)90067-2
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AN - SCOPUS:38249041941
SN - 0038-0717
VL - 18
SP - 315
EP - 319
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
IS - 3
ER -