Determination of Enantiomers of Amino Acids by Reversed Phase High Performance Liquid Chromatography

C. Gilon; R. Leshem; Eli Grushka

doi:10.1021/ac50058a011

Determination of Enantiomers of Amino Acids by Reversed Phase High Performance Liquid Chromatography

C. Gilon, R. Leshem, Eli Grushka^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

The chromatographic resolution of enantiomeric amino acids is accomplished on reversed phase columns using aqueous mobile phases containing the chiral reagent L-aspartylcyclohexylamide- Cu(II) [AspcHex-Cu(II)]. This reagent has the additional advantage that the copper ions allow the detection of nonaromatic amino acids at 230 nm. The separation seems to be a result of hydrophobic interactions between the cyclohexylamide moiety of the chiral additive and the side chain of the amino acids. The results indicate that the composition of the chiral reagent in the mobile phase is (AspcHex)₂Cu(II) and that the amino acids must replace one of the AspcHex for the resolution to occur.

Original language	English
Pages (from-to)	1206-1209
Number of pages	4
Journal	Analytical Chemistry
Volume	52
Issue number	8
DOIs	https://doi.org/10.1021/ac50058a011
State	Published - 1 Jun 1980

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title = "Determination of Enantiomers of Amino Acids by Reversed Phase High Performance Liquid Chromatography",

abstract = "The chromatographic resolution of enantiomeric amino acids is accomplished on reversed phase columns using aqueous mobile phases containing the chiral reagent L-aspartylcyclohexylamide- Cu(II) [AspcHex-Cu(II)]. This reagent has the additional advantage that the copper ions allow the detection of nonaromatic amino acids at 230 nm. The separation seems to be a result of hydrophobic interactions between the cyclohexylamide moiety of the chiral additive and the side chain of the amino acids. The results indicate that the composition of the chiral reagent in the mobile phase is (AspcHex)2Cu(II) and that the amino acids must replace one of the AspcHex for the resolution to occur.",

author = "C. Gilon and R. Leshem and Eli Grushka",

year = "1980",

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AU - Gilon, C.

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N2 - The chromatographic resolution of enantiomeric amino acids is accomplished on reversed phase columns using aqueous mobile phases containing the chiral reagent L-aspartylcyclohexylamide- Cu(II) [AspcHex-Cu(II)]. This reagent has the additional advantage that the copper ions allow the detection of nonaromatic amino acids at 230 nm. The separation seems to be a result of hydrophobic interactions between the cyclohexylamide moiety of the chiral additive and the side chain of the amino acids. The results indicate that the composition of the chiral reagent in the mobile phase is (AspcHex)2Cu(II) and that the amino acids must replace one of the AspcHex for the resolution to occur.

AB - The chromatographic resolution of enantiomeric amino acids is accomplished on reversed phase columns using aqueous mobile phases containing the chiral reagent L-aspartylcyclohexylamide- Cu(II) [AspcHex-Cu(II)]. This reagent has the additional advantage that the copper ions allow the detection of nonaromatic amino acids at 230 nm. The separation seems to be a result of hydrophobic interactions between the cyclohexylamide moiety of the chiral additive and the side chain of the amino acids. The results indicate that the composition of the chiral reagent in the mobile phase is (AspcHex)2Cu(II) and that the amino acids must replace one of the AspcHex for the resolution to occur.

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Determination of Enantiomers of Amino Acids by Reversed Phase High Performance Liquid Chromatography

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