DETERMINATION OF FeEDDHA IN SOILS AND FERTILIZERS BY ANION EXCHANGE CHROMATOGRAPHY.

The specific conditions for the quantitative analysis of FeEDDHA (ethylenedimine di-o-hydroxyphenylacetic acid) by high pressure liquid chromatography (HPLC) were studied and defined. Using a 3-cm anion exchange column and 5 mM H//2SO//4 plus 0. 01 mM Fe//2(SO//4)//3 as an eluent, FeEDDHA was separated into the dl-racemic and meso isomers. With the same column and 3 mM H//2SO//4 plus 50 mM Na//2SO//4 plus 0. 01 mM Fe//2(SO//4)//3, FeEDDHA eluted as a single peak. Standard calibration curves for FeEDDHA were obtained with regression coefficients of 0. 9999 at concentrations up to 0. 25 mM. The proposed method was used to determine FeEDDHA concentrations in water extracts of soils that were incubated with the compound.