Determination of immunoglobulin classes of specific antibodies secreted by single cells. A cluster formation assay

Michael Steinitz

doi:10.1016/0022-1759(80)90334-8

Determination of immunoglobulin classes of specific antibodies secreted by single cells. A cluster formation assay

Michael Steinitz^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A method is described to identify specific antibody-forming cells and their immunoglobulin classes by a multilayer cluster formation. Cells producing specific antibodies are mixed with erythrocytes coated with the corresponding antigen in presence of rabbit anti-human immunoglobulins. Clusters are formed only if the mixtures contain rabbit anti-serum against the immunoglobulin chains of the specific secreted antibody. Thus the immunoglobulin class of the specific antibody is assessed. For this study the cells of three EBV-transformed, antibody-producing human lymphoblastoid cell lines were used.

Original language	English
Pages (from-to)	95-102
Number of pages	8
Journal	Journal of Immunological Methods
Volume	38
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(80)90334-8
State	Published - 1980
Externally published	Yes

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10.1016/0022-1759(80)90334-8

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abstract = "A method is described to identify specific antibody-forming cells and their immunoglobulin classes by a multilayer cluster formation. Cells producing specific antibodies are mixed with erythrocytes coated with the corresponding antigen in presence of rabbit anti-human immunoglobulins. Clusters are formed only if the mixtures contain rabbit anti-serum against the immunoglobulin chains of the specific secreted antibody. Thus the immunoglobulin class of the specific antibody is assessed. For this study the cells of three EBV-transformed, antibody-producing human lymphoblastoid cell lines were used.",

author = "Michael Steinitz",

year = "1980",

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AB - A method is described to identify specific antibody-forming cells and their immunoglobulin classes by a multilayer cluster formation. Cells producing specific antibodies are mixed with erythrocytes coated with the corresponding antigen in presence of rabbit anti-human immunoglobulins. Clusters are formed only if the mixtures contain rabbit anti-serum against the immunoglobulin chains of the specific secreted antibody. Thus the immunoglobulin class of the specific antibody is assessed. For this study the cells of three EBV-transformed, antibody-producing human lymphoblastoid cell lines were used.

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ER -

Determination of immunoglobulin classes of specific antibodies secreted by single cells. A cluster formation assay

Abstract

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