Abstract
A method is described to identify specific antibody-forming cells and their immunoglobulin classes by a multilayer cluster formation. Cells producing specific antibodies are mixed with erythrocytes coated with the corresponding antigen in presence of rabbit anti-human immunoglobulins. Clusters are formed only if the mixtures contain rabbit anti-serum against the immunoglobulin chains of the specific secreted antibody. Thus the immunoglobulin class of the specific antibody is assessed. For this study the cells of three EBV-transformed, antibody-producing human lymphoblastoid cell lines were used.
| Original language | English |
|---|---|
| Pages (from-to) | 95-102 |
| Number of pages | 8 |
| Journal | Journal of Immunological Methods |
| Volume | 38 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 1980 |
| Externally published | Yes |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
-
SDG 3 Good Health and Well-being
Fingerprint
Dive into the research topics of 'Determination of immunoglobulin classes of specific antibodies secreted by single cells. A cluster formation assay'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver