Determination of 18O content of phosphate compounds - a novel method

J. Bar-Tana; O. Ben-Zeev; G. Rose; J. Deutshi

doi:10.1016/0304-4165(72)90134-1

Determination of ¹⁸O content of phosphate compounds - a novel method

J. Bar-Tana^*
, O. Ben-Zeev
, G. Rose
, J. Deutshi

^*Corresponding author for this work

Braun School of Public Health and Community Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Silylation of phosphate and phosphate-containing compounds provides a suitable means for the direct measurement of the ¹⁸O content of phosphate compounds. The silylation procedure is described for two model systems: ATP hydrolysis by H₂¹⁸O and ¹⁸O transfer from [carboxy-¹⁸O] acetic acid to AMP. An evaluation of the suggested procedure is presented.

Original language	English
Pages (from-to)	214-219
Number of pages	6
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	264
Issue number	1
DOIs	https://doi.org/10.1016/0304-4165(72)90134-1
State	Published - 30 Mar 1972

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title = "Determination of 18O content of phosphate compounds - a novel method",

abstract = "Silylation of phosphate and phosphate-containing compounds provides a suitable means for the direct measurement of the 18O content of phosphate compounds. The silylation procedure is described for two model systems: ATP hydrolysis by H218O and 18O transfer from [carboxy-18O] acetic acid to AMP. An evaluation of the suggested procedure is presented.",

author = "J. Bar-Tana and O. Ben-Zeev and G. Rose and J. Deutshi",

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AU - Bar-Tana, J.

AU - Ben-Zeev, O.

AU - Rose, G.

AU - Deutshi, J.

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Y1 - 1972/3/30

N2 - Silylation of phosphate and phosphate-containing compounds provides a suitable means for the direct measurement of the 18O content of phosphate compounds. The silylation procedure is described for two model systems: ATP hydrolysis by H218O and 18O transfer from [carboxy-18O] acetic acid to AMP. An evaluation of the suggested procedure is presented.

AB - Silylation of phosphate and phosphate-containing compounds provides a suitable means for the direct measurement of the 18O content of phosphate compounds. The silylation procedure is described for two model systems: ATP hydrolysis by H218O and 18O transfer from [carboxy-18O] acetic acid to AMP. An evaluation of the suggested procedure is presented.

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Determination of ¹⁸O content of phosphate compounds - a novel method

Abstract

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