Determining the sex of infanticide victims from the late roman era through ancient DNA analysis

Infanticide has since time immemorial been an accepted practice for disposing of unwanted infants. Archaeological evidence for infanticide was obtained in Ashkelon, where skeletal remains of some 100 neonates were discovered in a sewer, beneath a Roman bathhouse, which might have also served as a brothel. Written sources indicate that in ancient Roman society infanticide, especially of females, was commonly practised, but that females were occasionally saved and reared as courtesans. We performed DNA-based sex identification of the infant remains. Out of 43 left femurs tested 19 specimens provided results: 14 were found to be males and 5 females. The high frequency of males suggests selective preservation of females and that the infants may have been offspring of courtesans, serving in the bathhouse, supporting its use as a brothel.