Development of a Functional Backbone Cyclic Mimetic of the HIV-1 Tat Arginine-rich Motif

Assaf Friedler, Dorit Friedler, Nathan W. Luedtke, Yitzhak Tor, Abraham Loyter, Chaim Gilon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

We have used the backbone cyclic proteinomimetics approach to develop peptides that functionally mimic the arginine-rich motif (ARM) of the HIV-1 Tat protein. This consensus sequence serves both as a nuclear localization signal (NLS) and as an RNA binding domain. Based on the NMR structure of Tat, we have designed and synthesized a backbone cyclic ARM mimetic peptide library. The peptides were screened for their ability to mediate nuclear import of the corresponding BSA conjugates in permeabilized cells. One peptide, designated 'Tat11,' displayed active NLS properties. Nuclear import of Tat11-BSA was found to proceed by the same distinct pathway used by the Tat-NLS and not by the common importin α pathway, which is used by the SV40-NLS. Most of the Tat-derived backbone cyclic peptides display selective inhibitory activity as demonstrated by the inhibition of the nuclear import mediated by the Tat-NLS and not by the SV40-NLS. The Tat-ARM-derived peptides, including Tat-11, also inhibited binding of the HIV-1 Rev-ARM to its corresponding RNA element (Rev response element) with inhibition constants of 5 nM. Here we have shown for the first time (α) a functional mimetic of a protein sequence, which activates a nuclear import receptor and (b) a mimetic of a protein sequence with a dual functionality. Tat11 is a lead compound which can potentially inhibit the HIV-1 life cycle by a dual mechanism: inhibition of nuclear import and of RNA binding.

Original languageAmerican English
Pages (from-to)23783-23789
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number31
DOIs
StatePublished - 4 Aug 2000

Fingerprint

Dive into the research topics of 'Development of a Functional Backbone Cyclic Mimetic of the HIV-1 Tat Arginine-rich Motif'. Together they form a unique fingerprint.

Cite this