Development of a rapid, reliable screening method for pathogenicity of Colletotrichum species on strawberry seedlings

S. Freeman*, S. Horowitz, A. Zveibil, O. Yarden

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A rapid in vitro bioassay was developed, enabling large scale screening and isolation of nonpathogenic mutants of Colletotrichum acutatum and C. gloeosporioides on strawberry seedlings. Inoculation was carried out on 12 week-old (young) and 15 week-old (older) strawberry seedlings. A comparison was made between a foliar dip and a root soak method at 19°C and 25°C. Mortality of young seedlings was observed 4 days after inoculation, reaching 50% within 10 days using both techniques at 25°C. Mortality of older seedlings was delayed by 4 days compared to that in young seedlings with the root soak method. Disease progress decreased at 19°C. More than 980 REMI mutants were screened, resulting in five reduced-virulence isolates.

Original languageAmerican English
Pages (from-to)287-292
Number of pages6
JournalActa Horticulturae
Volume708
DOIs
StatePublished - 2006

Keywords

  • Anthracnose
  • Appressoria
  • Germination
  • Reduced virulence

Fingerprint

Dive into the research topics of 'Development of a rapid, reliable screening method for pathogenicity of Colletotrichum species on strawberry seedlings'. Together they form a unique fingerprint.

Cite this