Development of a transgenic early flowering pear (Pyrus communis L.) genotype by RNAi silencing of PcTFL1-1 and PcTFL1-2

Aviad Freiman, Lyudmila Shlizerman, Sara Golobovitch, Zeev Yablovitz, Raia Korchinsky, Yuval Cohen, Alon Samach, Elisabeth Chevreau, Pierre Marie Le Roux, Andrea Patocchi, Moshe A. Flaishman

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

Trees require a long maturation period, known as juvenile phase, before they can reproduce, complicating their genetic improvement as compared to annual plants. 'Spadona', one of the most important European pear (Pyrus communis L.) cultivars grown in Israel, has a very long juvenile period, up to 14 years, making breeding programs extremely slow. Progress in understanding the molecular basis of the transition to flowering has revealed genes that accelerate reproductive development when ectopically expressed in transgenic plants. A transgenic line of 'Spadona', named Early Flowering-Spadona (EF-Spa), was produced using a MdTFL1 RNAi cassette targeting the native pear genes PcTFL1-1 and PcTFL1-2. The transgenic line had three T-DNA insertions, one assigned to chromosome 2 and two to chromosome 14 PcTFL1-1 and PcTFL1-2 were completely silenced, and EF-Spa displayed an early flowering phenotype: flowers developed already in tissue culture and on most rooted plants 1-8 months after transfer to the greenhouse. EF-Spa developed solitary flowers from apical or lateral buds, reducing vegetative growth vigor. Pollination of EF-Spa trees generated normal-shaped fruits with viable F1 seeds. The greenhouse-grown transgenic F1 seedlings formed shoots and produced flowers 1-33 months after germination. Sequence analyses, of the non-transgenic F1 seedlings, demonstrated that this approach can be used to recover seedlings that have no trace of the T-DNA. Thus, the early flowering transgenic line EF-Spa obtained by PcTFL1 silencing provides an interesting tool to accelerate pear breeding.

Original languageAmerican English
Pages (from-to)1239-1251
Number of pages13
JournalPlanta
Volume235
Issue number6
DOIs
StatePublished - Jun 2012

Bibliographical note

Funding Information:
Acknowledgments This research was supported by the Israel Chief Scientist of the Ministry of Agriculture grant. We thank Yves Lespin-asse for providing the pollen of the pear cultivar p2714RR.

Keywords

  • Genetic transformation
  • Juvenility
  • MdTFL1
  • Plant breeding
  • Precocious flowering

Fingerprint

Dive into the research topics of 'Development of a transgenic early flowering pear (Pyrus communis L.) genotype by RNAi silencing of PcTFL1-1 and PcTFL1-2'. Together they form a unique fingerprint.

Cite this