Development of nanoscale structure in LAT-based signaling complexes

Valarie A. Barr, Eilon Sherman, Jason Yi, Itoro Akpan, Alexandre K. Rouquette-Jazdanian, Lawrence E. Samelson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The adapter molecule linker for activation of T cells (LAT) plays a crucial role in forming signaling complexes induced by stimulation of the T cell receptor (TCR). These multi-molecular complexes are dynamic structures that activate highly regulated signaling pathways. Previously, we have demonstrated nanoscale structure in LAT-based complexes where the adapter SLP-76 (also known as LCP2) localizes to the periphery of LAT clusters. In this study, we show that initially LAT and SLP-76 are randomly dispersed throughout the clusters that form upon TCR engagement. The segregation of LAT and SLP-76 develops near the end of the spreading process. The local concentration of LAT also increases at the same time. Both changes require TCR activation and an intact actin cytoskeleton. These results demonstrate that the nanoscale organization of LATbased signaling complexes is dynamic and indicates that different kinds of LAT-based complexes appear at different times during T cell activation.

Original languageAmerican English
Pages (from-to)4548-4562
Number of pages15
JournalJournal of Cell Science
Volume129
Issue number24
DOIs
StatePublished - 2016

Bibliographical note

Publisher Copyright:
© 2016. Published by The Company of Biologists Ltd.

Keywords

  • Microcluster
  • Nanostructure
  • Photoactivated localization microscopy
  • Signaling complex
  • Single-molecule localization microscopy
  • T cell activation

Fingerprint

Dive into the research topics of 'Development of nanoscale structure in LAT-based signaling complexes'. Together they form a unique fingerprint.

Cite this