Development of novel biosensor enzyme electrodes: Glucose oxidase multilayer arrays immobilized onto self‐assembled monolayers on electrodes

Amperometric biosensors under development are based on electron transfer communication between enzyme redox sites and electrodes. These require well‐organized networks of enzymes exhibiting long‐range electron transfer properties. An enzyme electrode is described that consists of glucose oxidase networks organized on a self‐assembled monolayer of functionalized thiols on gold electrodes. The protein array supports electrical communication with the electrode, and the resulting assembly can be used as a glucose biosensor.