Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

B. R. Berridge; J. D. Fuller; J. De Azavedo; D. E. Low; H. Bercovier; P. F. Frelier

doi:10.1128/jcm.36.9.2778-2781.1998

Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

B. R. Berridge^*, J. D. Fuller, J. De Azavedo, D. E. Low, H. Bercovier, P. F. Frelier

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.

Original language	English
Pages (from-to)	2778-2781
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	36
Issue number	9
DOIs	https://doi.org/10.1128/jcm.36.9.2778-2781.1998
State	Published - 1998
Externally published	Yes

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10.1128/jcm.36.9.2778-2781.1998

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abstract = "Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.",

author = "Berridge, {B. R.} and Fuller, {J. D.} and {De Azavedo}, J. and Low, {D. E.} and H. Bercovier and Frelier, {P. F.}",

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AU - Bercovier, H.

AU - Frelier, P. F.

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AB - Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.

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Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

Abstract

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