Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

B. R. Berridge; J. D. Fuller; J. De Azavedo; D. E. Low; H. Bercovier; P. F. Frelier

doi:10.1128/jcm.36.9.2778-2781.1998

Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

B. R. Berridge^*
, J. D. Fuller
, J. De Azavedo
, D. E. Low
, H. Bercovier
, P. F. Frelier

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.

Original language	English
Pages (from-to)	2778-2781
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	36
Issue number	9
DOIs	https://doi.org/10.1128/jcm.36.9.2778-2781.1998
State	Published - 1998
Externally published	Yes

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10.1128/jcm.36.9.2778-2781.1998

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abstract = "Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.",

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doi = "10.1128/jcm.36.9.2778-2781.1998",

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AU - Berridge, B. R.

AU - Fuller, J. D.

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AU - Low, D. E.

AU - Bercovier, H.

AU - Frelier, P. F.

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AB - Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.

UR - https://www.scopus.com/pages/publications/0031827865

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Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer

Abstract

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