Dexamethasone induces high-amplitude rhythms in preadipocytes, But hinders circadian expression in differentiated adipocytes

Maayan Barnea, Zecharia Madar, Oren Froy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Glucocorticoids induce circadian gene expression in cultured cells and change the phase of circadian gene expression in vivo. In addition, glucocorticoids induce differentiation of preadipocyte to adipocytes. We set out to test the effect of dexamethasone, a glucocorticoid receptor agonist, on circadian rhythms in 3T3-L1 differentiated adipocytes. Our results show that differentiated adipocytes exhibit robust circadian rhythms without dexamethasone. Dexamethasone induces phase changes and increases the amplitude of circadian gene expression in nondifferentiated 3T3-L1 preadipocytes. However, dexamethasone had an opposite effect on differentiated adipocytes, leading to low-amplitude circadian expression. In conclusion, although glucocorticoids reset circadian rhythms, once rhythms are reset, glucocorticoid administration hinders circadian expression.

Original languageAmerican English
Pages (from-to)837-842
Number of pages6
JournalChronobiology International
Volume30
Issue number6
DOIs
StatePublished - Jul 2013

Keywords

  • Adipocytes
  • Circadian rhythms
  • Clock
  • Dexamethasone
  • Obesity

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