Diagnostic and prognostic potential of antibodies against O-acetylated sialic acids in canine visceral leishmaniasis

Mitali Chatterjee, Gad Baneth, Charles L. Jaffe, Vineeta Sharma, Chitra Mandal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Employing bovine submaxillary mucin (BSM) as the coating agent, an enzyme-linked immunosorbent assay (BSM-ELISA) was developed to detect antibodies directed against O-acetylated sialic acids (O-AcSA) in canine visceral leishmaniasis (CVL). Serum samples were collected from 50 dogs previously screened by a parasite-ELISA to detect anti-leishmanial antibodies and designated as seropositive (n=30) and seronegative (n=20). The BSM-ELISA detected anti-O-AcSA antibodies in 29 out of 30 seropositive dogs and was negative in 15 out of 20 seronegative dogs; the sensitivity and specificity of the assay being 96.6% and 75%, respectively. Seven dogs from an endemic area in central Israel were longitudinally monitored for 15 months clinically, serologically and cultured for parasite. The levels of antibodies directed against O-AcSA increased with the appearance of clinical symptoms and/or seropositivity, disappeared when the disease was self-limiting as also with chemotherapeutic response and reappeared with relapse. The BSM-ELISA, therefore, represents a valuable tool for assessment of disease progression. Copyright (C) 1999 Elsevier Science B.V.

Original languageAmerican English
Pages (from-to)55-65
Number of pages11
JournalVeterinary Immunology and Immunopathology
Issue number1-2
StatePublished - 1 Sep 1999

Bibliographical note

Funding Information:
This research received support from the Department of Biotechnology, Government of India (MC, VS & CM), USAID grant-TA-REP-98-P8-140(GB) and Centre for Emerging Diseases (CLJ). V. Sharma is a recipient of a U.G.C. fellowship. We would like to express our sincere thanks to Mr. Ashish Mullick for his technical assistance.


  • Antibodies against O-acetylated sialic acids
  • Bovine submaxillary mucin
  • Canine visceral leishmaniasis
  • Serodiagnosis


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