Abstract
Employing bovine submaxillary mucin (BSM) as the coating agent, an enzyme-linked immunosorbent assay (BSM-ELISA) was developed to detect antibodies directed against O-acetylated sialic acids (O-AcSA) in canine visceral leishmaniasis (CVL). Serum samples were collected from 50 dogs previously screened by a parasite-ELISA to detect anti-leishmanial antibodies and designated as seropositive (n=30) and seronegative (n=20). The BSM-ELISA detected anti-O-AcSA antibodies in 29 out of 30 seropositive dogs and was negative in 15 out of 20 seronegative dogs; the sensitivity and specificity of the assay being 96.6% and 75%, respectively. Seven dogs from an endemic area in central Israel were longitudinally monitored for 15 months clinically, serologically and cultured for parasite. The levels of antibodies directed against O-AcSA increased with the appearance of clinical symptoms and/or seropositivity, disappeared when the disease was self-limiting as also with chemotherapeutic response and reappeared with relapse. The BSM-ELISA, therefore, represents a valuable tool for assessment of disease progression. Copyright (C) 1999 Elsevier Science B.V.
Original language | English |
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Pages (from-to) | 55-65 |
Number of pages | 11 |
Journal | Veterinary Immunology and Immunopathology |
Volume | 70 |
Issue number | 1-2 |
DOIs | |
State | Published - 1 Sep 1999 |
Bibliographical note
Funding Information:This research received support from the Department of Biotechnology, Government of India (MC, VS & CM), USAID grant-TA-REP-98-P8-140(GB) and Centre for Emerging Diseases (CLJ). V. Sharma is a recipient of a U.G.C. fellowship. We would like to express our sincere thanks to Mr. Ashish Mullick for his technical assistance.
Keywords
- Antibodies against O-acetylated sialic acids
- Bovine submaxillary mucin
- Canine visceral leishmaniasis
- Serodiagnosis