Dielectric relaxation, rotational diffusion and the heat denaturation transition in aqueous solutions of RNAse A

Yu D. Feldman*, V. D. Fedotov

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The heat denaturation transition of pancreatic RNAse A was investigated by time-domain spectroscopy. In the temperature interval 5-90°C the protein molecule may be in three states: native, temperature denaturated and unfolded. The heat denaturation process occurs in two stages: In the first stage the system behaves as a macromolecule, maintaining its globular structure and increasing its volume two times and its dipole moment 1.5 times; in the second stage the transition into a statistical coil state Occurs.

Original languageEnglish
Pages (from-to)309-312
Number of pages4
JournalChemical Physics Letters
Volume143
Issue number3
DOIs
StatePublished - 15 Jan 1988
Externally publishedYes

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