Abstract
Gene rearrangement in the immune system is always preceded by DNA demethylation and increased chromatin accessibility. Using a model system in which rearrangement of the endogenous immunoglobulin κ locus is prevented, we demonstrate that these epigenetic and chromatin changes actually occur on one allele with a higher probability than the other. It may be this process that, together with feedback inhibition, serves as the basis for allelic exclusion.
Original language | English |
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Pages (from-to) | 5255-5261 |
Number of pages | 7 |
Journal | EMBO Journal |
Volume | 21 |
Issue number | 19 |
DOIs | |
State | Published - 1 Oct 2002 |
Keywords
- Chromatin
- DNA methylation
- DNase I sensitivity
- Double strand breaks
- V(D)J rearrangement