Differential changes in GPR55 during microglial cell activation

Maciej Pietr, Ewa Kozela, Rivka Levy, Neta Rimmerman, Yi Hsing Lin, Nephi Stella, Zvi Vogel*, Ana Juknat

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

We examined how lipopolysaccharide (LPS) and interferon gamma (IFN-γ), known to differentially activate microglia, affect the expression of G protein-coupled receptor 55 (GPR55), a novel cannabinoid receptor. We found that GPR55 mRNA is significantly expressed in both primary mouse microglia and the BV-2 mouse microglial cell line, and that LPS down-regulates this message. Conversely, IFN-γ slightly decreases GPR55 mRNA in primary microglia, while it upregulates this message in BV-2 cells. Moreover, the GPR55 agonist, lysophosphatidylinositol, increases ERK phosphorylation in BV-2 stimulated with IFN-γ, in correlation with the increased amount of GPR55 mRNA. Remarkably, these stimuli-induced changes in GPR55 expression are similar to those observed with CB2-R, suggesting that both receptors might be involved in neuroinflammation and that their expression is concomitantly controlled by the state of microglial activation.

Original languageAmerican English
Pages (from-to)2071-2076
Number of pages6
JournalFEBS Letters
Volume583
Issue number12
DOIs
StatePublished - 18 Jun 2009
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the Dr. Miriam and Sheldon G. Adelson Program for Nerve Regeneration and Repair, the Nella and Leon Benoziyo Center for Neurosciences and the Israeli Ministry of Health (Z.V.) and by NIDA (N. S.). A.J. is supported by the Israeli Ministry of Absorption.

Keywords

  • CB receptor
  • Cannabinoid
  • ERK phosphorylation
  • G protein-coupled receptor 55
  • Interferon gamma
  • Lipopolysaccharide
  • Lysophosphatidylinositol
  • Microglia

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