TY - JOUR
T1 - Differential effect of ethacrynic acid on microsomal Mg2+-ATPase
AU - Wald, Hannah
AU - Gutman, Yehuda
AU - Czaczkes, Walter
PY - 1979
Y1 - 1979
N2 - The effect of ethacrynic acid (EA) was studied on microsomal ATPase in various tissues of the rat and in kidneys of different species. Inhibition of Na,K-ATPase in microsomes of kidney medulla and cortex was observed only at 5 × 10-3 M EA. At concentrations of 10-5-10-4 M, EA caused some enhancement of Na,K-ATPase activity. Microsomal Mg-ATPase of kidney medulla and cortex was inhibited by EA at concentrations of 10-5-10-3 M in various rodents (rat, mouse, guinea-pig and rabbit) as well as in human and cat kidney. EA showed the same differential effect on kidney microsomal Mg-ATPase when microsomes were prepared without treatment with desoxycholate, when chloride was replaced by sulfate in the medium. EA inhibited preferentially Mg-ATPase vs Na, K-ATPase in micro-somes of rat brain and rat submaxillary gland.
AB - The effect of ethacrynic acid (EA) was studied on microsomal ATPase in various tissues of the rat and in kidneys of different species. Inhibition of Na,K-ATPase in microsomes of kidney medulla and cortex was observed only at 5 × 10-3 M EA. At concentrations of 10-5-10-4 M, EA caused some enhancement of Na,K-ATPase activity. Microsomal Mg-ATPase of kidney medulla and cortex was inhibited by EA at concentrations of 10-5-10-3 M in various rodents (rat, mouse, guinea-pig and rabbit) as well as in human and cat kidney. EA showed the same differential effect on kidney microsomal Mg-ATPase when microsomes were prepared without treatment with desoxycholate, when chloride was replaced by sulfate in the medium. EA inhibited preferentially Mg-ATPase vs Na, K-ATPase in micro-somes of rat brain and rat submaxillary gland.
UR - http://www.scopus.com/inward/record.url?scp=0018344268&partnerID=8YFLogxK
U2 - 10.1016/0006-2952(79)90522-7
DO - 10.1016/0006-2952(79)90522-7
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C2 - 154892
AN - SCOPUS:0018344268
SN - 0006-2952
VL - 28
SP - 321
EP - 325
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
IS - 2
ER -
