Differential effect of ethacrynic acid on microsomal Mg2+-ATPase

Hannah Wald; Yehuda Gutman; Walter Czaczkes

doi:10.1016/0006-2952(79)90522-7

Differential effect of ethacrynic acid on microsomal Mg²⁺-ATPase

Hannah Wald^*
, Yehuda Gutman
, Walter Czaczkes

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The effect of ethacrynic acid (EA) was studied on microsomal ATPase in various tissues of the rat and in kidneys of different species. Inhibition of Na,K-ATPase in microsomes of kidney medulla and cortex was observed only at 5 × 10^-3 M EA. At concentrations of 10^-5-10^-4 M, EA caused some enhancement of Na,K-ATPase activity. Microsomal Mg-ATPase of kidney medulla and cortex was inhibited by EA at concentrations of 10^-5-10^-3 M in various rodents (rat, mouse, guinea-pig and rabbit) as well as in human and cat kidney. EA showed the same differential effect on kidney microsomal Mg-ATPase when microsomes were prepared without treatment with desoxycholate, when chloride was replaced by sulfate in the medium. EA inhibited preferentially Mg-ATPase vs Na, K-ATPase in micro-somes of rat brain and rat submaxillary gland.

Original language	English
Pages (from-to)	321-325
Number of pages	5
Journal	Biochemical Pharmacology
Volume	28
Issue number	2
DOIs	https://doi.org/10.1016/0006-2952(79)90522-7
State	Published - 1979

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title = "Differential effect of ethacrynic acid on microsomal Mg2+-ATPase",

abstract = "The effect of ethacrynic acid (EA) was studied on microsomal ATPase in various tissues of the rat and in kidneys of different species. Inhibition of Na,K-ATPase in microsomes of kidney medulla and cortex was observed only at 5 × 10-3 M EA. At concentrations of 10-5-10-4 M, EA caused some enhancement of Na,K-ATPase activity. Microsomal Mg-ATPase of kidney medulla and cortex was inhibited by EA at concentrations of 10-5-10-3 M in various rodents (rat, mouse, guinea-pig and rabbit) as well as in human and cat kidney. EA showed the same differential effect on kidney microsomal Mg-ATPase when microsomes were prepared without treatment with desoxycholate, when chloride was replaced by sulfate in the medium. EA inhibited preferentially Mg-ATPase vs Na, K-ATPase in micro-somes of rat brain and rat submaxillary gland.",

author = "Hannah Wald and Yehuda Gutman and Walter Czaczkes",

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doi = "10.1016/0006-2952(79)90522-7",

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T1 - Differential effect of ethacrynic acid on microsomal Mg2+-ATPase

AU - Wald, Hannah

AU - Gutman, Yehuda

AU - Czaczkes, Walter

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Y1 - 1979

N2 - The effect of ethacrynic acid (EA) was studied on microsomal ATPase in various tissues of the rat and in kidneys of different species. Inhibition of Na,K-ATPase in microsomes of kidney medulla and cortex was observed only at 5 × 10-3 M EA. At concentrations of 10-5-10-4 M, EA caused some enhancement of Na,K-ATPase activity. Microsomal Mg-ATPase of kidney medulla and cortex was inhibited by EA at concentrations of 10-5-10-3 M in various rodents (rat, mouse, guinea-pig and rabbit) as well as in human and cat kidney. EA showed the same differential effect on kidney microsomal Mg-ATPase when microsomes were prepared without treatment with desoxycholate, when chloride was replaced by sulfate in the medium. EA inhibited preferentially Mg-ATPase vs Na, K-ATPase in micro-somes of rat brain and rat submaxillary gland.

AB - The effect of ethacrynic acid (EA) was studied on microsomal ATPase in various tissues of the rat and in kidneys of different species. Inhibition of Na,K-ATPase in microsomes of kidney medulla and cortex was observed only at 5 × 10-3 M EA. At concentrations of 10-5-10-4 M, EA caused some enhancement of Na,K-ATPase activity. Microsomal Mg-ATPase of kidney medulla and cortex was inhibited by EA at concentrations of 10-5-10-3 M in various rodents (rat, mouse, guinea-pig and rabbit) as well as in human and cat kidney. EA showed the same differential effect on kidney microsomal Mg-ATPase when microsomes were prepared without treatment with desoxycholate, when chloride was replaced by sulfate in the medium. EA inhibited preferentially Mg-ATPase vs Na, K-ATPase in micro-somes of rat brain and rat submaxillary gland.

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JO - Biochemical Pharmacology

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Differential effect of ethacrynic acid on microsomal Mg²⁺-ATPase

Abstract

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