Differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys

Stephanie Walter, Andrea Rasche, Andrés Moreira-Soto, Stephanie Pfaender, Magda Bletsa, Victor Max Corman, Alvaro Aguilar-Setien, Fernando García-Lacy, Aymeric Hans, Daniel Todt, Gerhard Schuler, Anat Shnaiderman-Torban, Amir Steinman, Cristina Roncoroni, Vincenzo Veneziano, Nikolina Rusenova, Nikolay Sandev, Anton Rusenov, Dimitrinka Zapryanova, Ignacio García-BocanegraJoerg Jores, Augusto Carluccio, Maria Cristina Veronesi, Jessika M.V. Cavalleri, Christian Drosten, Philippe Lemey, Eike Steinmann*, Jan Felix Drexler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


The hepatitis C virus (HCV) is a major human pathogen. Genetically related viruses in animals suggest a zoonotic origin of HCV. The closest relative of HCV is found in horses (termed equine hepacivirus [EqHV]). However, low EqHV genetic diversity implies relatively recent acquisition of EqHV by horses, making a derivation of HCV from EqHV unlikely. To unravel the EqHV evolutionary history within equid sister species, we analyzed 829 donkeys and 53 mules sampled in nine European, Asian, African, and American countries by molecular and serologic tools for EqHV infection. Antibodies were found in 278 animals (31.5%), and viral RNA was found in 3 animals (0.3%), all of which were simultaneously seropositive. A low RNA prevalence in spite of high seroprevalence suggests a predominance of acute infection, a possible difference from the mostly chronic hepacivirus infection pattern seen in horses and humans. Limitation of transmission due to short courses of infection may explain the existence of entirely seronegative groups of animals. Donkey and horse EqHV strains were paraphyletic and 97.5 to 98.2% identical in their translated polyprotein sequences, making virus/host cospeciation unlikely. Evolutionary reconstructions supported host switches of EqHV between horses and donkeys without the involvement of adaptive evolution. Global admixture of donkey and horse hepaciviruses was compatible with anthropogenic alterations of EqHV ecology. In summary, our findings do not support EqHV as the origin of the significantly more diversified HCV. Identification of a host system with predominantly acute hepacivirus infection may enable new insights into the chronic infection pattern associated with HCV.

Original languageAmerican English
Article numbere01711-16
JournalJournal of Virology
Issue number1
StatePublished - 2017

Bibliographical note

Publisher Copyright:
© 2016 American Society for Microbiology.


  • Donkey
  • Equine hepacivirus
  • Evolution
  • Hepatitis C virus
  • Pathogenesis


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