Differential tissue-specific expression of NtAQP1 in Arabidopsis thaliana reveals a role for this protein in stomatal and mesophyll conductance of CO2 under standard and salt-stress conditions.

Nir Sade; Alexander Gallé; Jaume Flexas; Stephen Lerner; Gadi Peleg; Adi Yaaran; Menachem Moshelion

doi:10.1007/s00425-013-1988-8

Differential tissue-specific expression of NtAQP1 in Arabidopsis thaliana reveals a role for this protein in stomatal and mesophyll conductance of CO₂ under standard and salt-stress conditions.

Nir Sade^*, Alexander Gallé, Jaume Flexas, Stephen Lerner, Gadi Peleg, Adi Yaaran, Menachem Moshelion

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

The regulation of plant hydraulic conductance and gas conductance involves a number of different morphological, physiological and molecular mechanisms working in harmony. At the molecular level, aquaporins play a key role in the transport of water, as well as CO₂, through cell membranes. Yet, their tissue-related function, which controls whole-plant gas exchange and water relations, is less understood. In this study, we examined the tissue-specific effects of the stress-induced tobacco Aquaporin1 (NtAQP1), which functions as both a water and CO₂ channel, on whole-plant behavior. In tobacco and tomato plants, constitutive overexpression of NtAQP1 increased net photosynthesis (A(N)), mesophyll CO₂ conductance (g(m)) and stomatal conductance (g(s)) and, under stress, increased root hydraulic conductivity (L(pr)) as well. Our results revealed that NtAQP1 that is specifically expressed in the mesophyll tissue plays an important role in increasing both A(N) and g(m). Moreover, targeting NtAQP1 expression to the cells of the vascular envelope significantly improved the plants' stress response. Surprisingly, NtAQP1 expression in the guard cells did not have a significant effect under any of the tested conditions. The tissue-specific involvement of NtAQP1 in hydraulic and gas conductance via the interaction between the vasculature and the stomata is discussed.

Original language	English
Pages (from-to)	357-366
Number of pages	10
Journal	Planta
Volume	239
Issue number	2
DOIs	https://doi.org/10.1007/s00425-013-1988-8
State	Published - Feb 2014

Bibliographical note

Funding Information:
Acknowledgments This study was supported by a grant from the Rehovot-Hohenheim partnership program and the Israel Science Foundation, Jerusalem (ISF; Grant # 1311/12) to MM. JF acknowledges the support of Plan Nacional (Spain) project grant MECOME (BFU2011-23294). We thank Dr. Orit Edelbaum and Professor Shmuel Wolf (Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot, Israel), Dr. Einat Sadot and Dr. Mohamad Abu-Abied (Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel), Professor Yuval Eshed (Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel) and Professor Ralf Kaldenhoff (Department of Biology, Applied Plant Sciences, Tech-nische Universität Darmstadt, Darmstadt, Germany) for supplying the different constructs and plant materials. Eduard Belausov (Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel) helped with microscopy imaging.

Access to Document

10.1007/s00425-013-1988-8

Cite this

@article{41369742b6c043b9857d7ae6d1f23611,

title = "Differential tissue-specific expression of NtAQP1 in Arabidopsis thaliana reveals a role for this protein in stomatal and mesophyll conductance of CO2 under standard and salt-stress conditions.",

abstract = "The regulation of plant hydraulic conductance and gas conductance involves a number of different morphological, physiological and molecular mechanisms working in harmony. At the molecular level, aquaporins play a key role in the transport of water, as well as CO2, through cell membranes. Yet, their tissue-related function, which controls whole-plant gas exchange and water relations, is less understood. In this study, we examined the tissue-specific effects of the stress-induced tobacco Aquaporin1 (NtAQP1), which functions as both a water and CO2 channel, on whole-plant behavior. In tobacco and tomato plants, constitutive overexpression of NtAQP1 increased net photosynthesis (A(N)), mesophyll CO2 conductance (g(m)) and stomatal conductance (g(s)) and, under stress, increased root hydraulic conductivity (L(pr)) as well. Our results revealed that NtAQP1 that is specifically expressed in the mesophyll tissue plays an important role in increasing both A(N) and g(m). Moreover, targeting NtAQP1 expression to the cells of the vascular envelope significantly improved the plants' stress response. Surprisingly, NtAQP1 expression in the guard cells did not have a significant effect under any of the tested conditions. The tissue-specific involvement of NtAQP1 in hydraulic and gas conductance via the interaction between the vasculature and the stomata is discussed.",

author = "Nir Sade and Alexander Gall{\'e} and Jaume Flexas and Stephen Lerner and Gadi Peleg and Adi Yaaran and Menachem Moshelion",

note = "Funding Information: Acknowledgments This study was supported by a grant from the Rehovot-Hohenheim partnership program and the Israel Science Foundation, Jerusalem (ISF; Grant # 1311/12) to MM. JF acknowledges the support of Plan Nacional (Spain) project grant MECOME (BFU2011-23294). We thank Dr. Orit Edelbaum and Professor Shmuel Wolf (Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot, Israel), Dr. Einat Sadot and Dr. Mohamad Abu-Abied (Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel), Professor Yuval Eshed (Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel) and Professor Ralf Kaldenhoff (Department of Biology, Applied Plant Sciences, Tech-nische Universit{\"a}t Darmstadt, Darmstadt, Germany) for supplying the different constructs and plant materials. Eduard Belausov (Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel) helped with microscopy imaging.",

year = "2014",

month = feb,

doi = "10.1007/s00425-013-1988-8",

language = "אנגלית",

volume = "239",

pages = "357--366",

journal = "Planta",

issn = "0032-0935",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "2",

}

TY - JOUR

T1 - Differential tissue-specific expression of NtAQP1 in Arabidopsis thaliana reveals a role for this protein in stomatal and mesophyll conductance of CO2 under standard and salt-stress conditions.

AU - Sade, Nir

AU - Gallé, Alexander

AU - Flexas, Jaume

AU - Lerner, Stephen

AU - Peleg, Gadi

AU - Yaaran, Adi

AU - Moshelion, Menachem

N1 - Funding Information: Acknowledgments This study was supported by a grant from the Rehovot-Hohenheim partnership program and the Israel Science Foundation, Jerusalem (ISF; Grant # 1311/12) to MM. JF acknowledges the support of Plan Nacional (Spain) project grant MECOME (BFU2011-23294). We thank Dr. Orit Edelbaum and Professor Shmuel Wolf (Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Hebrew University of Jerusalem, Rehovot, Israel), Dr. Einat Sadot and Dr. Mohamad Abu-Abied (Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel), Professor Yuval Eshed (Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel) and Professor Ralf Kaldenhoff (Department of Biology, Applied Plant Sciences, Tech-nische Universität Darmstadt, Darmstadt, Germany) for supplying the different constructs and plant materials. Eduard Belausov (Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel) helped with microscopy imaging.

PY - 2014/2

Y1 - 2014/2

N2 - The regulation of plant hydraulic conductance and gas conductance involves a number of different morphological, physiological and molecular mechanisms working in harmony. At the molecular level, aquaporins play a key role in the transport of water, as well as CO2, through cell membranes. Yet, their tissue-related function, which controls whole-plant gas exchange and water relations, is less understood. In this study, we examined the tissue-specific effects of the stress-induced tobacco Aquaporin1 (NtAQP1), which functions as both a water and CO2 channel, on whole-plant behavior. In tobacco and tomato plants, constitutive overexpression of NtAQP1 increased net photosynthesis (A(N)), mesophyll CO2 conductance (g(m)) and stomatal conductance (g(s)) and, under stress, increased root hydraulic conductivity (L(pr)) as well. Our results revealed that NtAQP1 that is specifically expressed in the mesophyll tissue plays an important role in increasing both A(N) and g(m). Moreover, targeting NtAQP1 expression to the cells of the vascular envelope significantly improved the plants' stress response. Surprisingly, NtAQP1 expression in the guard cells did not have a significant effect under any of the tested conditions. The tissue-specific involvement of NtAQP1 in hydraulic and gas conductance via the interaction between the vasculature and the stomata is discussed.

AB - The regulation of plant hydraulic conductance and gas conductance involves a number of different morphological, physiological and molecular mechanisms working in harmony. At the molecular level, aquaporins play a key role in the transport of water, as well as CO2, through cell membranes. Yet, their tissue-related function, which controls whole-plant gas exchange and water relations, is less understood. In this study, we examined the tissue-specific effects of the stress-induced tobacco Aquaporin1 (NtAQP1), which functions as both a water and CO2 channel, on whole-plant behavior. In tobacco and tomato plants, constitutive overexpression of NtAQP1 increased net photosynthesis (A(N)), mesophyll CO2 conductance (g(m)) and stomatal conductance (g(s)) and, under stress, increased root hydraulic conductivity (L(pr)) as well. Our results revealed that NtAQP1 that is specifically expressed in the mesophyll tissue plays an important role in increasing both A(N) and g(m). Moreover, targeting NtAQP1 expression to the cells of the vascular envelope significantly improved the plants' stress response. Surprisingly, NtAQP1 expression in the guard cells did not have a significant effect under any of the tested conditions. The tissue-specific involvement of NtAQP1 in hydraulic and gas conductance via the interaction between the vasculature and the stomata is discussed.

UR - http://www.scopus.com/inward/record.url?scp=84905057107&partnerID=8YFLogxK

U2 - 10.1007/s00425-013-1988-8

DO - 10.1007/s00425-013-1988-8

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 24170337

AN - SCOPUS:84905057107

SN - 0032-0935

VL - 239

SP - 357

EP - 366

JO - Planta

JF - Planta

IS - 2

ER -

Differential tissue-specific expression of NtAQP1 in Arabidopsis thaliana reveals a role for this protein in stomatal and mesophyll conductance of CO₂ under standard and salt-stress conditions.

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this