Differential transcriptional profiles mediated by exposure to the cannabinoids cannabidiol and Δ 9-tetrahydrocannabinol in BV-2 microglial cells

Ana Juknat, Maciej Pietr, Ewa Kozela, Neta Rimmerman, Rivka Levy, Giovanni Coppola, Daniel Geschwind, Zvi Vogel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

69 Scopus citations


BACKGROUND AND PURPOSE: Apart from their effects on mood and reward, cannabinoids exert beneficial actions such as neuroprotection and attenuation of inflammation. The immunosuppressive activity of cannabinoids has been well established. However, the underlying mechanisms are largely unknown. We previously showed that the psychoactive cannabinoid Δ 9- tetrahydrocannabinol (THC) and the non-psychoactive cannabidiol (CBD) differ in their anti-inflammatory signalling pathways. EXPERIMENTAL APPROACH: To characterize the transcriptional effects of CBD and THC, we treated BV-2 microglial cells with these compounds and performed comparative microarray analysis using the Illumina MouseRef-8 BeadChip platform. Ingenuity Pathway Analysis was performed to identify functional subsets of genes and networks regulated by CBD and/or THC. KEY RESULTS: Overall, CBD altered the expression of many more genes; from the 1298 transcripts found to be differentially regulated by the treatments, 680 gene probe sets were up-regulated by CBD and 58 by THC, and 524 gene products were down-regulated by CBD and only 36 by THC. CBD-specific gene expression profile showed changes associated with oxidative stress and glutathione depletion, normally occurring under nutrient limiting conditions or proteasome inhibition and involving the GCN2/eIF2α/p8/ATF4/ CHOP-TRIB3 pathway. Furthermore, CBD-stimulated genes were shown to be controlled by nuclear factors known to be involved in the regulation of stress response and inflammation, mainly via the (EpRE/ARE)-Nrf2/ATF4 system and the Nrf2/Hmox1 axis. CONCLUSIONS AND IMPLICATIONS: These observations indicated that CBD, but much less than THC, induced a cellular stress response in microglial cells and suggested that this effect could underlie its anti-inflammatory activity. LINKED ARTICLES: This article is part of a themed section on Cannabinoids in Biology and Medicine. To view the other articles in this section visit http://dx.doi.org/10.1111/bph.2012.165.issue-8. To view Part I of Cannabinoids in Biology and Medicine visit http://dx.doi.org/10.1111/bph.2011. 163.issue-7.

Original languageAmerican English
Pages (from-to)2512-2528
Number of pages17
JournalBritish Journal of Pharmacology
Issue number8
StatePublished - Apr 2012
Externally publishedYes


  • Cannabidiol
  • Gene expression
  • Glutathione depletion
  • Nuclear factor-erythroid 2-related factor 2
  • Oxidative stress
  • Δ -tetrahydrocannabinol


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