TY - JOUR
T1 - Differentiation of dissociated-reconstituted epiblasts of the chick under the influence of a normal hypoblast
AU - Mitrani, Eduardo
AU - Eyal-Giladi, Hefzibah
PY - 1984
Y1 - 1984
N2 - A cell suspension of chick epiblast cells cultured under defined conditions to form a flat disk, can differentiate and generate axial embryonic structures when covered with a primary hypoblast. Macroscopically identifiable axes developed in 26 out of 33 cases. In all cases axes developed in a direction consistent with the posteroanterior polarity of the normal hypoblast. Almost invariably the epiblast cells differentiated into ectoderm, neural plates or tubes, and endoderm. In some cases typical primitive streaks were found, sometimes accompanied by signs of axial mesoderm, whereas in other cases the primitive streaks seemed to regress. In the absence of a hypoblast no differentiation of neural tissue or any signs of axial development were observed.
AB - A cell suspension of chick epiblast cells cultured under defined conditions to form a flat disk, can differentiate and generate axial embryonic structures when covered with a primary hypoblast. Macroscopically identifiable axes developed in 26 out of 33 cases. In all cases axes developed in a direction consistent with the posteroanterior polarity of the normal hypoblast. Almost invariably the epiblast cells differentiated into ectoderm, neural plates or tubes, and endoderm. In some cases typical primitive streaks were found, sometimes accompanied by signs of axial mesoderm, whereas in other cases the primitive streaks seemed to regress. In the absence of a hypoblast no differentiation of neural tissue or any signs of axial development were observed.
UR - http://www.scopus.com/inward/record.url?scp=0021267785&partnerID=8YFLogxK
U2 - 10.1111/j.1432-0436.1984.tb01382.x
DO - 10.1111/j.1432-0436.1984.tb01382.x
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AN - SCOPUS:0021267785
SN - 0301-4681
VL - 26
SP - 107
EP - 111
JO - Differentiation
JF - Differentiation
IS - 1-3
ER -