Differentiation of dissociated-reconstituted epiblasts of the chick under the influence of a normal hypoblast

Eduardo Mitrani*, Hefzibah Eyal-Giladi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A cell suspension of chick epiblast cells cultured under defined conditions to form a flat disk, can differentiate and generate axial embryonic structures when covered with a primary hypoblast. Macroscopically identifiable axes developed in 26 out of 33 cases. In all cases axes developed in a direction consistent with the posteroanterior polarity of the normal hypoblast. Almost invariably the epiblast cells differentiated into ectoderm, neural plates or tubes, and endoderm. In some cases typical primitive streaks were found, sometimes accompanied by signs of axial mesoderm, whereas in other cases the primitive streaks seemed to regress. In the absence of a hypoblast no differentiation of neural tissue or any signs of axial development were observed.

Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalDifferentiation
Volume26
Issue number1-3
DOIs
StatePublished - 1984

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