Differentiation of schistosoma haematobium from related schistosomes by PCR amplifying an inter-repeat sequence

I. Abbasi, C.H. King, R.F. Sturrock, C. Kariuki, E. Muchiri, J. Hamburger

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Schistosoma haematobium infects nearly 150 million people, primarily in Africa, and is transmitted by select species of local bulinid snails. These snails can host other related trematode species as well, so that effective detection and monitoring of snails infected with S. haematobium requires a successful differentiation between S. haematobium and any closely related schistosome species. To enable differential detection of S. haematobium DNA by simple polymerase chain reaction (PCR), we designed and tested primer pairs from numerous newly identified Schistosoma DNA repeat sequences. However, all pairs tested were found unsuitable for this purpose. Differentiation of S. haematobium from S. bovis, S. mattheei, S. curassoni, and S. intercalatum (but not from S. margrebowiei) was ultimately accomplished by PCR using one primer from a newly identified repeat, Sh110, and a second primer from a known schistosomal splice-leader sequence. For evaluation of residual S. haematobium transmission after control interventions, this differentiation tool will enable accurate monitoring of infected snails in areas where S. haematobium is sympatric with the most prevalent other schistosome species. Copyright © 2007 by The American Society of Tropical Medicine and Hygiene.
Original languageEnglish
Pages (from-to)950-955
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number5
StatePublished - 2007

Bibliographical note

Cited By :25

Export Date: 30 January 2023


Correspondence Address: Abbasi, I.; Department of Parasitology, PO Box 12272, Jerusalem 91120, Israel; email: ibrahim@md.huji.ac.il

Molecular Sequence Numbers: GENBANK: DQ831697, DQ831698, DQ831699, DQ831700, DQ831701, DQ831702, DQ831703, DQ831704, DQ831705, DQ831706, DQ831696;

Chemicals/CAS: DNA, 9007-49-2; DNA Primers; DNA, Helminth; Deoxyribonucleases, Type II Site-Specific,; endodeoxyribonuclease AluI, 3.1.21.-; endodeoxyribonuclease RsaI, 3.1.21.-

Funding details: National Institute of Allergy and Infectious Diseases, NIAID, U01AI045473

Funding details: Fogarty International Center, FIC, R01TW001543


  • DNA
  • repetitive DNA
  • endodeoxyribonuclease AluI
  • endodeoxyribonuclease RsaI
  • helminth DNA
  • primer DNA
  • type II site specific deoxyribonuclease
  • unclassified drug
  • article
  • Bulinus
  • DNA extraction
  • DNA sequence
  • dot hybridization
  • Kenya
  • nonhuman
  • nucleotide sequence
  • parasite identification
  • parasite transmission
  • polymerase chain reaction
  • Schistosoma bovis
  • schistosoma curassoni
  • Schistosoma hematobium
  • Schistosoma intercalatum
  • schistosoma margrebowiei
  • schistosoma mattheei
  • sensitivity and specificity
  • Southern blotting
  • animal
  • chemistry
  • classification
  • genetics
  • metabolism
  • methodology
  • molecular genetics
  • nucleotide repeat
  • parasitology
  • Schistosoma
  • Gastropoda
  • Planorbidae
  • Schistosoma curassoni
  • Schistosoma haematobium
  • Schistosoma margrebowiei
  • Schistosoma mattheei
  • Trematoda
  • Animals
  • Base Sequence
  • Deoxyribonucleases, Type II Site-Specific
  • DNA Primers
  • DNA, Helminth
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Repetitive Sequences, Nucleic Acid
  • Sensitivity and Specificity


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