Differentiation of schistosoma haematobium from related schistosomes by PCR amplifying an inter-repeat sequence

Ibrahim Abbasi*, Charles H. King, Robert F. Sturrock, Curtis Kariuki, Eric Muchiri, Joseph Hamburger

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Schistosoma haematobium infects nearly 150 million people, primarily in Africa, and is transmitted by select species of local bulinid snails. These snails can host other related trematode species as well, so that effective detection and monitoring of snails infected with S. haematobium requires a successful differentiation between S. haematobium and any closely related schistosome species. To enable differential detection of S. haematobium DNA by simple polymerase chain reaction (PCR), we designed and tested primer pairs from numerous newly identified Schistosoma DNA repeat sequences. However, all pairs tested were found unsuitable for this purpose. Differentiation of S. haematobium from S. bovis, S. mattheei, S. curassoni, and S. intercalatum (but not from S. margrebowiei) was ultimately accomplished by PCR using one primer from a newly identified repeat, Sh110, and a second primer from a known schistosomal splice-leader sequence. For evaluation of residual S. haematobium transmission after control interventions, this differentiation tool will enable accurate monitoring of infected snails in areas where S. haematobium is sympatric with the most prevalent other schistosome species.

Original languageAmerican English
Pages (from-to)950-955
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume76
Issue number5
DOIs
StatePublished - May 2007

Fingerprint

Dive into the research topics of 'Differentiation of schistosoma haematobium from related schistosomes by PCR amplifying an inter-repeat sequence'. Together they form a unique fingerprint.

Cite this