Diffusion cannot govern the discharge of neurotransmitter in fast synapses

In the present work we show that diffusion cannot provide the observed fast discharge of neurotransmitter from a synaptic vesicle during neurotransmitter release, mainly because it is not sufficiently rapid nor is it sufficiently temperature-dependent. Modeling the discharge from the vesicle into the cleft as a continuous point source, we have determined that discharge should occur in 50–75 microseconds, to provide the observed high concentrations of transmitter at the critical zone.