TY - JOUR
T1 - Diffusion coefficients of mixed micelles of Triton X-100 and sphingomyelin and of sonicated sphingomyelin liposomes, measured by autocorrelation spectroscopy of Rayleigh scattered light
AU - Cooper, V. G.
AU - Yedgar, S.
AU - Barenholz, Y.
PY - 1974/8/21
Y1 - 1974/8/21
N2 - Autocorrelation spectroscopy of Rayleigh scattered light has been used to measure the diffusion coefficients of mixed micelles of Triton X-100 with sphingomyelin. Sphingomyelin is a water-insoluble lipid. It forms a solution of mixed micelles only for molar fractions of Triton X-100 above 0.32. Over the range of Triton X-100 molar fractions from 0.32-1.0 the diffusion coefficient increases from 2.6·10-7-4.5·10-7 cm2/s, indicating that the increased concentration ratio of the detergent systematically reduces the size of the mixed micelles. Diffusion coefficients of sonicated liposomes of sphingomyelin were also measured. For specimens of unhydrogenated and partially hydrogenated sphingomyelin, respective diffusion coefficients of 8.8·10-8 and 3.1·10-8 cm2/s were obtained. The corresponding Stokes radii for the two specimens are 250 and 700 Å, respectively.
AB - Autocorrelation spectroscopy of Rayleigh scattered light has been used to measure the diffusion coefficients of mixed micelles of Triton X-100 with sphingomyelin. Sphingomyelin is a water-insoluble lipid. It forms a solution of mixed micelles only for molar fractions of Triton X-100 above 0.32. Over the range of Triton X-100 molar fractions from 0.32-1.0 the diffusion coefficient increases from 2.6·10-7-4.5·10-7 cm2/s, indicating that the increased concentration ratio of the detergent systematically reduces the size of the mixed micelles. Diffusion coefficients of sonicated liposomes of sphingomyelin were also measured. For specimens of unhydrogenated and partially hydrogenated sphingomyelin, respective diffusion coefficients of 8.8·10-8 and 3.1·10-8 cm2/s were obtained. The corresponding Stokes radii for the two specimens are 250 and 700 Å, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0016189256&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(74)90008-X
DO - 10.1016/0005-2736(74)90008-X
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C2 - 4851390
AN - SCOPUS:0016189256
SN - 0005-2736
VL - 363
SP - 86
EP - 97
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 1
ER -