Diffusion coefficients of mixed micelles of Triton X-100 and sphingomyelin and of sonicated sphingomyelin liposomes, measured by autocorrelation spectroscopy of Rayleigh scattered light

V. G. Cooper*, S. Yedgar, Y. Barenholz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Autocorrelation spectroscopy of Rayleigh scattered light has been used to measure the diffusion coefficients of mixed micelles of Triton X-100 with sphingomyelin. Sphingomyelin is a water-insoluble lipid. It forms a solution of mixed micelles only for molar fractions of Triton X-100 above 0.32. Over the range of Triton X-100 molar fractions from 0.32-1.0 the diffusion coefficient increases from 2.6·10-7-4.5·10-7 cm2/s, indicating that the increased concentration ratio of the detergent systematically reduces the size of the mixed micelles. Diffusion coefficients of sonicated liposomes of sphingomyelin were also measured. For specimens of unhydrogenated and partially hydrogenated sphingomyelin, respective diffusion coefficients of 8.8·10-8 and 3.1·10-8 cm2/s were obtained. The corresponding Stokes radii for the two specimens are 250 and 700 Å, respectively.

Original languageEnglish
Pages (from-to)86-97
Number of pages12
JournalBiochimica et Biophysica Acta - Biomembranes
Volume363
Issue number1
DOIs
StatePublished - 21 Aug 1974

Fingerprint

Dive into the research topics of 'Diffusion coefficients of mixed micelles of Triton X-100 and sphingomyelin and of sonicated sphingomyelin liposomes, measured by autocorrelation spectroscopy of Rayleigh scattered light'. Together they form a unique fingerprint.

Cite this