Dimers as Intermediates in the Assembly of Tetrameric Proteins: A Study on Lactate Dehydrogenase Isozymes

Alexander Levitzki; Hana Tenenbaum

doi:10.1002/ijch.197400026

Dimers as Intermediates in the Assembly of Tetrameric Proteins: A Study on Lactate Dehydrogenase Isozymes

Alexander Levitzki^*
, Hana Tenenbaum

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The renaturation kinetics of pig heart, pig muscle, beef heart and beef muscle lactic dehydrogenases was studied. It was found that in all four cases the major intermediate in the assembly process is a lactate dehydrogenase dimer. It was found that NAD⁺ and NADH influence dramatically the kinetics of renaturation, probably by binding to a partially refolded subunit prior to the assembly to the fully renatured tetramer.

Original language	English
Pages (from-to)	327-337
Number of pages	11
Journal	Israel Journal of Chemistry
Volume	12
Issue number	1-2
DOIs	https://doi.org/10.1002/ijch.197400026
State	Published - 1974
Externally published	Yes

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title = "Dimers as Intermediates in the Assembly of Tetrameric Proteins: A Study on Lactate Dehydrogenase Isozymes",

abstract = "The renaturation kinetics of pig heart, pig muscle, beef heart and beef muscle lactic dehydrogenases was studied. It was found that in all four cases the major intermediate in the assembly process is a lactate dehydrogenase dimer. It was found that NAD+ and NADH influence dramatically the kinetics of renaturation, probably by binding to a partially refolded subunit prior to the assembly to the fully renatured tetramer.",

author = "Alexander Levitzki and Hana Tenenbaum",

year = "1974",

doi = "10.1002/ijch.197400026",

language = "אנגלית",

volume = "12",

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journal = "Israel Journal of Chemistry",

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publisher = "Wiley-Blackwell",

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AU - Tenenbaum, Hana

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Y1 - 1974

N2 - The renaturation kinetics of pig heart, pig muscle, beef heart and beef muscle lactic dehydrogenases was studied. It was found that in all four cases the major intermediate in the assembly process is a lactate dehydrogenase dimer. It was found that NAD+ and NADH influence dramatically the kinetics of renaturation, probably by binding to a partially refolded subunit prior to the assembly to the fully renatured tetramer.

AB - The renaturation kinetics of pig heart, pig muscle, beef heart and beef muscle lactic dehydrogenases was studied. It was found that in all four cases the major intermediate in the assembly process is a lactate dehydrogenase dimer. It was found that NAD+ and NADH influence dramatically the kinetics of renaturation, probably by binding to a partially refolded subunit prior to the assembly to the fully renatured tetramer.

UR - https://www.scopus.com/pages/publications/84987313515

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DO - 10.1002/ijch.197400026

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SN - 0021-2148

VL - 12

SP - 327

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JO - Israel Journal of Chemistry

JF - Israel Journal of Chemistry

IS - 1-2

ER -

Dimers as Intermediates in the Assembly of Tetrameric Proteins: A Study on Lactate Dehydrogenase Isozymes

Abstract

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