Direct detection of molecular biorecognition by dipole sensing mechanism

Ilya Goykhman, Nina Korbakov, Carmen Bartic, Gustaaf Borghs, Micha E. Spira, Joseph Shappir, Shlomo Yitzchaik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

This work investigates the feasibility of transducing molecular-recognition events into a measurable potentiometric signal. It is shown for the first time that biorecognition of acetylcholine (ACh) can be translated to conformational changes in the enzyme, acetylcholine-esterase (AChE), which in turn induces a measurable change in surface potential. Our results show that a highly sensitive detector for ACh can be obtained by the dilute assembly of AChE on a floating gate derived field effect transistor (FG-FET). A wide concentration range response is observed for ACh (10-2-10-9M) and for the inhibitor carbamylcholine CCh (10-6-10-11M). These enhanced sensitivities are modeled theoretically and explained by the combined response of the device to local pH changes and molecular dipole variations due to the enzyme-substrate recognition event.

Original languageAmerican English
Pages (from-to)4788-4794
Number of pages7
JournalJournal of the American Chemical Society
Volume131
Issue number13
DOIs
StatePublished - 8 Apr 2009

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