Direct detection of molecular biorecognition by dipole sensing mechanism

Ilya Goykhman; Nina Korbakov; Carmen Bartic; Gustaaf Borghs; Micha E. Spira; Joseph Shappir; Shlomo Yitzchaik

doi:10.1021/ja809051p

Direct detection of molecular biorecognition by dipole sensing mechanism

Ilya Goykhman, Nina Korbakov, Carmen Bartic, Gustaaf Borghs, Micha E. Spira, Joseph Shappir, Shlomo Yitzchaik^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

This work investigates the feasibility of transducing molecular-recognition events into a measurable potentiometric signal. It is shown for the first time that biorecognition of acetylcholine (ACh) can be translated to conformational changes in the enzyme, acetylcholine-esterase (AChE), which in turn induces a measurable change in surface potential. Our results show that a highly sensitive detector for ACh can be obtained by the dilute assembly of AChE on a floating gate derived field effect transistor (FG-FET). A wide concentration range response is observed for ACh (10^-2-10^-9M) and for the inhibitor carbamylcholine CCh (10^-6-10^-11M). These enhanced sensitivities are modeled theoretically and explained by the combined response of the device to local pH changes and molecular dipole variations due to the enzyme-substrate recognition event.

Original language	English
Pages (from-to)	4788-4794
Number of pages	7
Journal	Journal of the American Chemical Society
Volume	131
Issue number	13
DOIs	https://doi.org/10.1021/ja809051p
State	Published - 8 Apr 2009

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abstract = "This work investigates the feasibility of transducing molecular-recognition events into a measurable potentiometric signal. It is shown for the first time that biorecognition of acetylcholine (ACh) can be translated to conformational changes in the enzyme, acetylcholine-esterase (AChE), which in turn induces a measurable change in surface potential. Our results show that a highly sensitive detector for ACh can be obtained by the dilute assembly of AChE on a floating gate derived field effect transistor (FG-FET). A wide concentration range response is observed for ACh (10-2-10-9M) and for the inhibitor carbamylcholine CCh (10-6-10-11M). These enhanced sensitivities are modeled theoretically and explained by the combined response of the device to local pH changes and molecular dipole variations due to the enzyme-substrate recognition event.",

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AU - Goykhman, Ilya

AU - Korbakov, Nina

AU - Bartic, Carmen

AU - Borghs, Gustaaf

AU - Spira, Micha E.

AU - Shappir, Joseph

AU - Yitzchaik, Shlomo

PY - 2009/4/8

Y1 - 2009/4/8

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AB - This work investigates the feasibility of transducing molecular-recognition events into a measurable potentiometric signal. It is shown for the first time that biorecognition of acetylcholine (ACh) can be translated to conformational changes in the enzyme, acetylcholine-esterase (AChE), which in turn induces a measurable change in surface potential. Our results show that a highly sensitive detector for ACh can be obtained by the dilute assembly of AChE on a floating gate derived field effect transistor (FG-FET). A wide concentration range response is observed for ACh (10-2-10-9M) and for the inhibitor carbamylcholine CCh (10-6-10-11M). These enhanced sensitivities are modeled theoretically and explained by the combined response of the device to local pH changes and molecular dipole variations due to the enzyme-substrate recognition event.

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Direct detection of molecular biorecognition by dipole sensing mechanism

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