Direct enzyme-substrate affinity determination by real-time hyperpolarized 13C-MRS

L. J. Friesen-Waldner; C. N. Wiens; T. P. Wade; K. Thind; K. P. Sinclair; Y. Hovav; J. M. Gomori; J. Sosna; C. A. McKenzie; R. Katz-Brull

doi:10.1039/c4cc05418k

Direct enzyme-substrate affinity determination by real-time hyperpolarized ¹³C-MRS

L. J. Friesen-Waldner
, C. N. Wiens
, T. P. Wade
, K. Thind
, K. P. Sinclair
, Y. Hovav
, J. M. Gomori
, J. Sosna
, C. A. McKenzie
, R. Katz-Brull^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D₄, 1-¹³C]choline ¹³C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (K_m), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 μM were detected at a temporal resolution of 1 s.

Original language	English
Pages (from-to)	13801-13804
Number of pages	4
Journal	Chemical Communications
Volume	50
Issue number	89
DOIs	https://doi.org/10.1039/c4cc05418k
State	Published - 14 Oct 2014
Externally published	Yes

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title = "Direct enzyme-substrate affinity determination by real-time hyperpolarized 13C-MRS",

abstract = "A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D4, 1-13C]choline 13C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (Km), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 μM were detected at a temporal resolution of 1 s.",

author = "Friesen-Waldner, \{L. J.\} and Wiens, \{C. N.\} and Wade, \{T. P.\} and K. Thind and Sinclair, \{K. P.\} and Y. Hovav and Gomori, \{J. M.\} and J. Sosna and McKenzie, \{C. A.\} and R. Katz-Brull",

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doi = "10.1039/c4cc05418k",

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AU - Friesen-Waldner, L. J.

AU - Wiens, C. N.

AU - Wade, T. P.

AU - Thind, K.

AU - Sinclair, K. P.

AU - Hovav, Y.

AU - Gomori, J. M.

AU - Sosna, J.

AU - McKenzie, C. A.

AU - Katz-Brull, R.

PY - 2014/10/14

Y1 - 2014/10/14

N2 - A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D4, 1-13C]choline 13C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (Km), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 μM were detected at a temporal resolution of 1 s.

AB - A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D4, 1-13C]choline 13C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (Km), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 μM were detected at a temporal resolution of 1 s.

UR - https://www.scopus.com/pages/publications/84908010491

U2 - 10.1039/c4cc05418k

DO - 10.1039/c4cc05418k

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C2 - 25253534

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SN - 1359-7345

VL - 50

SP - 13801

EP - 13804

JO - Chemical Communications

JF - Chemical Communications

IS - 89

ER -

Direct enzyme-substrate affinity determination by real-time hyperpolarized ¹³C-MRS

Abstract

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