Directly transfected langerin+ dermal dendritic cells potentiate CD8+ T cell responses following intradermal plasmid DNA immunization

Mazal Elnekave, Karina Furmanov, Itay Nudel, Moran Arizon, Björn E. Clausen, Avi Hai Hovav*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Dendritic cells (DCs) play a critical role in CD8+ T cell priming following DNA vaccination. In contrast to other DNA injection routes or immunization with viral vectors, Ag presentation is delayed following needle injection of plasmid DNA into the skin. The contribution of various skin DC subsets to this process is not known. In this study, we show that dermal CD11c+ cells are the most important transgene-expressing cells following immunization. Using langerin- diphtheria toxin receptor mice we demonstrated that langerin+ dermal DCs (Ln+dDCs) were crucial for generating an optimal CD8+ T cell response. Blocking migration of skin cells to the lymph node (LN) ablated immunogenicity, suggesting that migration of dDC subsets to the LN is essential for generating immunity. This migration generated a weak Ag-presenting activity in vivo until day 5 postimmunization, which then increased dramatically. We further found that Ln+dDCs and dDCs were the only DC populations directly presenting Ag to CD8+ T cells ex vivo during the initial 8-d period postimmunization. This activity changed on the following days, when both skin DCs and LN-resident DCs were able to present Ag to CD8+ T cells. Taken together, our in vivo and ex vivo results suggest that activation of CD8+ T cells following intradermal plasmid DNA immunization depends on directly transfected Ln+dDCs and dDCs. Moreover, the type of DCs presenting Ag changed over time, with Ln+dDCs playing the major role in potentiating the initial CD8+ T cell response.

Original languageAmerican English
Pages (from-to)3463-3471
Number of pages9
JournalJournal of Immunology
Volume185
Issue number6
DOIs
StatePublished - 15 Sep 2010

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