TY - JOUR
T1 - Discovery of microRNAs and other small RNAs in solid tumors
AU - Meiri, Eti
AU - Levy, Asaf
AU - Benjamin, Hila
AU - Ben-David, Miriam
AU - Cohen, Lahav
AU - Dov, Avital
AU - Dromi, Nir
AU - Elyakim, Eran
AU - Yerushalmi, Noga
AU - Zion, Orit
AU - Lithwick-Yanai, Gila
AU - Sitbon, Einat
N1 - Funding Information:
Funding for open access charge: Internal funding.
PY - 2010/5/18
Y1 - 2010/5/18
N2 - MicroRNAs (miRNAs) are ~22-nt long, non-coding RNAs that regulate gene silencing. It is known that many human miRNAs are deregulated in numerous types of tumors. Here we report the sequencing of small RNAs (17-25 nt) from 23 breast, bladder, colon and lung tumor samples using high throughput sequencing. We identified 49 novel miRNA and miR-sized small RNAs. We further validated the expression of 10 novel small RNAs in 31 different types of blood, normal and tumor tissue samples using two independent platforms, namely microarray and RT-PCR. Some of the novel sequences show a large difference in expression between tumor and tumor-adjacent tissues, between different tumor stages, or between different tumor types. We also report the identification of novel small RNA classes in human: highly expressed small RNA derived from Y-RNA and endogenous siRNA. Finally, we identified dozens of new miRNA sequence variants that demonstrate the existence of miRNA-related SNP or post-transcriptional modifications. Our work extends the current knowledge of the tumor small RNA transcriptome and provides novel candidates for molecular biomarkers and drug targets.
AB - MicroRNAs (miRNAs) are ~22-nt long, non-coding RNAs that regulate gene silencing. It is known that many human miRNAs are deregulated in numerous types of tumors. Here we report the sequencing of small RNAs (17-25 nt) from 23 breast, bladder, colon and lung tumor samples using high throughput sequencing. We identified 49 novel miRNA and miR-sized small RNAs. We further validated the expression of 10 novel small RNAs in 31 different types of blood, normal and tumor tissue samples using two independent platforms, namely microarray and RT-PCR. Some of the novel sequences show a large difference in expression between tumor and tumor-adjacent tissues, between different tumor stages, or between different tumor types. We also report the identification of novel small RNA classes in human: highly expressed small RNA derived from Y-RNA and endogenous siRNA. Finally, we identified dozens of new miRNA sequence variants that demonstrate the existence of miRNA-related SNP or post-transcriptional modifications. Our work extends the current knowledge of the tumor small RNA transcriptome and provides novel candidates for molecular biomarkers and drug targets.
UR - http://www.scopus.com/inward/record.url?scp=78049446142&partnerID=8YFLogxK
U2 - 10.1093/nar/gkq376
DO - 10.1093/nar/gkq376
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C2 - 20483914
AN - SCOPUS:78049446142
SN - 0305-1048
VL - 38
SP - 6234
EP - 6246
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 18
M1 - gkq376
ER -