Diseased leaf lyophilization: a method for long‐term prevention of loss of virulence in phytopathogenic bacteria

Y. Bashan*, Y. Okon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A technique for long‐term preservation of phytopathogenic bacteria is described. It is based on selective multiplication of the bacterial pathogen in host tissue, disinfection of leaf surfaces to reduce contamination, lyophilization of leaves, and storage under dry conditions at ‐ 80°C. With this technique, the pathogenicity of large numbers of the pathogens Pseudomonas syringae pv. tomato and Xanthomonas campestris pv. vesicatoria was maintained for 4 years.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalJournal of Applied Bacteriology
Volume61
Issue number2
DOIs
StatePublished - Aug 1986

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