TY - JOUR
T1 - Disruption of microtubules in living cells by tyrphostin AG-1714
AU - Volberg, Tova
AU - Bershadsky, Alexander D.
AU - Elbaum, Michael
AU - Gazit, Aviv
AU - Levitzki, Alexander
AU - Geiger, Benjamin
PY - 2000
Y1 - 2000
N2 - Tyrphostin AG-1714 and several related molecules with the general structure of nitro-benzene malononitrile (BMN) disrupt microtubules in a large variety of cultured cells. This process can be inhibited by the stabilization of microtubules with taxol or by pretreatment of the cells with pervanadate, which inhibits tyrosine phosphatases and increases the overall levels of phosphotyrosine in cells. Unlike other microtubule-disrupting drugs such as nocodazole or colchicine, tyrphostin AG-1714 does not interfere with microtubule polymerization or stability in vitro, suggesting that the effect of this tyrphostin on microtubules is indirect. These results imply an involvement of protein tyrosine phosphorylation in the regulation of overall microtubule dynamics. Tyrphostins of AG-1714 type could thus be powerful tools for the identification of such microtubule regulatory pathways. (C) 2000 Wiley-Liss, Inc.
AB - Tyrphostin AG-1714 and several related molecules with the general structure of nitro-benzene malononitrile (BMN) disrupt microtubules in a large variety of cultured cells. This process can be inhibited by the stabilization of microtubules with taxol or by pretreatment of the cells with pervanadate, which inhibits tyrosine phosphatases and increases the overall levels of phosphotyrosine in cells. Unlike other microtubule-disrupting drugs such as nocodazole or colchicine, tyrphostin AG-1714 does not interfere with microtubule polymerization or stability in vitro, suggesting that the effect of this tyrphostin on microtubules is indirect. These results imply an involvement of protein tyrosine phosphorylation in the regulation of overall microtubule dynamics. Tyrphostins of AG-1714 type could thus be powerful tools for the identification of such microtubule regulatory pathways. (C) 2000 Wiley-Liss, Inc.
KW - Microtubule dynamics
KW - Pervanadate
KW - Taxol
KW - Tyrosine phosphorylation
KW - Tyrphostins
UR - https://www.scopus.com/pages/publications/0034059503
U2 - 10.1002/(SICI)1097-0169(200003)45:3<223::AID-CM5>3.0.CO;2-Q
DO - 10.1002/(SICI)1097-0169(200003)45:3<223::AID-CM5>3.0.CO;2-Q
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C2 - 10706777
AN - SCOPUS:0034059503
SN - 0886-1544
VL - 45
SP - 223
EP - 234
JO - Cell Motility and the Cytoskeleton
JF - Cell Motility and the Cytoskeleton
IS - 3
ER -