Dissecting the contribution of microtubule behaviour in adventitious root induction

Mohamad Abu-Abied, Oksana Rogovoy, Inna Mordehaev, Marina Grumberg, Rivka Elbaum, Geoffrey O. Wasteneys, Einat Sadot*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Induction of adventitious roots (ARs) in recalcitrant plants often culminates in cell division and callus formation rather than root differentiation. Evidence is provided here to suggest that microtubules (MTs) play a role in the shift from cell division to cell differentiation during AR induction. First, it was found that fewer ARs form in the temperature-sensitive mutant mor1-1, in which the MT-associated protein MOR1 is mutated, and in bot1-1, in which the MT-severing protein katanin is mutated. In the two latter mutants, MT dynamics and form are perturbed. By contrast, the number of ARs increased in RIC1-OX3 plants, in which MT bundling is enhanced and katanin is activated. In addition, any1 plants in which cell walls are perturbed made more ARs than wild-type plants. MT perturbations during AR induction in mor1-1 or in wild-type hypocotyls treated with oryzalin led to the formation of amorphous clusters of cells reminiscent of callus. In these cells a specific pattern of polarized light retardation by the cell walls was lost. PIN1 polarization and auxin maxima were hampered and differentiation of the epidermis was inhibited. It is concluded that a fine-tuned crosstalk between MTs, cell walls, and auxin transport is required for proper AR induction.

Original languageAmerican English
Pages (from-to)2813-2824
Number of pages12
JournalJournal of Experimental Botany
Volume66
Issue number9
DOIs
StatePublished - 1 May 2015

Bibliographical note

Publisher Copyright:
© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Keywords

  • Adventitious roots
  • Arabidopsis
  • auxin
  • cell wall
  • microtubule
  • oryzalin

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