Dissection of the Functional Surface of an Anti-insect Excitatory Toxin Illuminates a Putative "Hot Spot" Common to All Scorpion β-Toxins Affecting Na+ Channels

Lior Cohen, Izhar Karbat, Nicolas Gilles, Oren Froy, Gerardo Corzo, Ruthie Angelovici, Dalia Gordon*, Michael Gurevitz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Scorpion β-toxins affect the activation of voltage-sensitive sodium channels (NaChs). Although these toxins have been instrumental in the study of channel gating and architecture, little is known about their active sites. By using an efficient system for the production of recombinant toxins, we analyzed by point mutagenesis the entire surface of the β-toxin, Bj-xtrIT, an anti-insect selective excitatory toxin from the scorpion Buthotus judaicus. Each toxin mutant was purified and analyzed using toxicity and binding assays, as well as by circular dichroism spectroscopy to discern the differences among mutations that caused structural changes and those that specifically affected bioactivity. This analysis highlighted a functional discontinuous surface of 1405 Å2, which was composed of a number of non-polar and three charged amino acids clustered around the main α-helical motif and the C-tail. Among the charged residues, Glu30 is a center of a putative "hot spot" in the toxin-receptor binding-interface and is shielded from bulk solvent by a hydrophobic "gasket" (Tyr26 and Val34). Comparison of the Bj-xtrIT structure with that of other β-toxins that are active on mammals suggests that the hot spot and an adjacent non-polar region are spatially conserved. These results highlight for the first time structural elements that constitute a putative "pharmacophore" involved in the interaction of β-toxins with receptor site-4 on NaChs. Furthermore, the unique structure of the C-terminal region most likely determines the specificity of excitatory toxins for insect NaChs.

Original languageEnglish
Pages (from-to)8206-8211
Number of pages6
JournalJournal of Biological Chemistry
Volume279
Issue number9
DOIs
StatePublished - 27 Feb 2004

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