TY - JOUR
T1 - Distinct constitutive and low-CO2-induced CO2 uptake systems in cyanobacteria
T2 - Genes involved and their phylogenetic relationship with homologous genes in other organisms
AU - Shibata, Mari
AU - Ohkawa, Hiroshi
AU - Kaneko, Takakazu
AU - Fukuzawa, Hideya
AU - Tabata, Satoshi
AU - Kaplan, Aaron
AU - Ogawa, Teruo
PY - 2001/9/25
Y1 - 2001/9/25
N2 - Cyanobacteria possess a CO2-concentating mechanism that involves active CO2 uptake and HCO3- transport. For CO2 uptake, we have identified two systems in the cyanobacterium Synechocystis sp. strain PCC 6803, one induced at low CO2 and one constitutive. The low CO2-induced system showed higher maximal activity and higher affinity for CO2 than the constitutive system. On the basis of speculation that separate NAD(P)H dehydrogenase complexes were essential for each of these systems, we reasoned that inactivation of one system would allow selection of mutants defective in the other. Thus, mutants unable to grow at pH 7.0 in air were recovered after transformation of a ΔndhD3 mutant with a transposon-bearing library. Four of them had tags within slr1302 (designated cupB), a homologue of sll1734 (cupA), which is cotranscribed with ndhF3 and ndhD3. The ΔcupB, ΔndhD4, and ΔndhF4 mutants showed CO2-uptake characteristics of the low CO2-induced system observed in wild type. In contrast, mutants ΔcupA, ΔndhD3, and ΔndhF3 showed characteristics of the constitutive CO2-uptake system. Double mutants impaired in one component of each of the systems were unable to take up CO2 and required high CO2 for growth. Phylogenetic analysis indicated that the ndhD3/ndhD4-, ndhF3/ndhF4-, and cupA/cupB-type genes are present only in cyanobacteria. Most of the cyanobacterial strains studied possess the ndhD3/ndhD4-, ndhF3/ndhF4-, and cupA/cupB-type genes in pairs. Thus, the two types of NAD(P)H dehydrogenase complexes essential for low CO2-induced and constitutive CO2-uptake systems associated with the NdhD3/NdhF3/CupA-homologues and NdhD4/NdhF4/CupB-homologues, respectively, appear to be present in these cyanobacterial strains but not in other organisms.
AB - Cyanobacteria possess a CO2-concentating mechanism that involves active CO2 uptake and HCO3- transport. For CO2 uptake, we have identified two systems in the cyanobacterium Synechocystis sp. strain PCC 6803, one induced at low CO2 and one constitutive. The low CO2-induced system showed higher maximal activity and higher affinity for CO2 than the constitutive system. On the basis of speculation that separate NAD(P)H dehydrogenase complexes were essential for each of these systems, we reasoned that inactivation of one system would allow selection of mutants defective in the other. Thus, mutants unable to grow at pH 7.0 in air were recovered after transformation of a ΔndhD3 mutant with a transposon-bearing library. Four of them had tags within slr1302 (designated cupB), a homologue of sll1734 (cupA), which is cotranscribed with ndhF3 and ndhD3. The ΔcupB, ΔndhD4, and ΔndhF4 mutants showed CO2-uptake characteristics of the low CO2-induced system observed in wild type. In contrast, mutants ΔcupA, ΔndhD3, and ΔndhF3 showed characteristics of the constitutive CO2-uptake system. Double mutants impaired in one component of each of the systems were unable to take up CO2 and required high CO2 for growth. Phylogenetic analysis indicated that the ndhD3/ndhD4-, ndhF3/ndhF4-, and cupA/cupB-type genes are present only in cyanobacteria. Most of the cyanobacterial strains studied possess the ndhD3/ndhD4-, ndhF3/ndhF4-, and cupA/cupB-type genes in pairs. Thus, the two types of NAD(P)H dehydrogenase complexes essential for low CO2-induced and constitutive CO2-uptake systems associated with the NdhD3/NdhF3/CupA-homologues and NdhD4/NdhF4/CupB-homologues, respectively, appear to be present in these cyanobacterial strains but not in other organisms.
KW - Affinity to CO
KW - CO-concentrating mechanism
KW - Constitutive CO uptake
KW - NAD(P)H dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=0035949508&partnerID=8YFLogxK
U2 - 10.1073/pnas.191258298
DO - 10.1073/pnas.191258298
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C2 - 11562454
AN - SCOPUS:0035949508
SN - 0027-8424
VL - 98
SP - 11789
EP - 11794
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 20
ER -