Distinct roles for PP1 and PP2A in the Neurospora circadian clock

Yuhong Yang, Qun He, Ping Cheng, Philip Wrage, Oded Yarden, Yi Liu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

102 Scopus citations


Phosphorylation of the Neurospora circadian clock protein FREQUENCY by several kinases promotes its degradation and is important for the function of the circadian feedback loop. Here, we show that FRQ is less stable in a ppp-1 (catalytic subunit of PP1) mutant, resulting in its advanced phase and short period. In contrast, FRQ stability is not altered in a rgb-1 (a regulatory subunit of PP2A) mutant, but levels of frq protein and mRNA are low, resulting in a low-amplitude and long-period oscillation of the clock. Furthermore, PP1 and PP2A expressed in Neurospora can dephosphorylate the endogenous FRQ in vitro, suggesting that these two phosphatases may differentially regulate FRQ and, consequently, the behavior of the circadian clock.

Original languageAmerican English
Pages (from-to)255-260
Number of pages6
JournalGenes and Development
Issue number3
StatePublished - 1 Feb 2004


  • Frequency
  • PP1
  • PP2A
  • Phosphatase
  • Phosphorylation


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