TY - JOUR
T1 - Distinction of species and strains of mycoplasmas (Mollicutes) by genomic DNA fingerprints with an rRNA gene probe
AU - Yogev, D.
AU - Halachmi, D.
AU - Kenny, G. E.
AU - Razin, S.
PY - 1988
Y1 - 1988
N2 - Genomic fingerprints of Acholeplasma laidlawii, Mycoplasma hominis, and Mycoplasma pneumoniae strains were obtained by Southern blot hybridization of the digested mycoplasmal DNAs with an rRNA gene probe. The hybridization patterns revealed genotypic heterogeneity among A. laidlawii and M. hominis strains and a remarkable degree of homogeneity among M. pneumoniae strains isolated from pneumonia patients during a 10-year period. Genomic fingerprints with the rRNA gene probe can thus serve as indicators of intraspecies genetic homogeneity or heterogeneity and can provide a new, sensitive tool for strain identification with a potential for application in epidemiology.
AB - Genomic fingerprints of Acholeplasma laidlawii, Mycoplasma hominis, and Mycoplasma pneumoniae strains were obtained by Southern blot hybridization of the digested mycoplasmal DNAs with an rRNA gene probe. The hybridization patterns revealed genotypic heterogeneity among A. laidlawii and M. hominis strains and a remarkable degree of homogeneity among M. pneumoniae strains isolated from pneumonia patients during a 10-year period. Genomic fingerprints with the rRNA gene probe can thus serve as indicators of intraspecies genetic homogeneity or heterogeneity and can provide a new, sensitive tool for strain identification with a potential for application in epidemiology.
UR - http://www.scopus.com/inward/record.url?scp=0023916891&partnerID=8YFLogxK
U2 - 10.1128/jcm.26.6.1198-1201.1988
DO - 10.1128/jcm.26.6.1198-1201.1988
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C2 - 3384931
AN - SCOPUS:0023916891
SN - 0095-1137
VL - 26
SP - 1198
EP - 1201
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 6
ER -
