Disturbance of the bacterial cell wall specifically interferes with biofilm formation

Tabitha Bucher, Yaara Oppenheimer-Shaanan, Alon Savidor, Zohar Bloom-Ackermann, Ilana Kolodkin-Gal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

In nature, bacteria communicate via chemical cues and establish complex communities referred to as biofilms, wherein cells are held together by an extracellular matrix. Much research is focusing on small molecules that manipulate and prevent biofilm assembly by modifying cellular signalling pathways. However, the bacterial cell envelope, presenting the interface between bacterial cells and their surroundings, is largely overlooked. In our study, we identified specific targets within the biosynthesis pathways of the different cell wall components (peptidoglycan, wall teichoic acids and teichuronic acids) hampering biofilm formation and the anchoring of the extracellular matrix with a minimal effect on planktonic growth. In addition, we provide convincing evidence that biofilm hampering by transglycosylation inhibitors and D-Leucine triggers a highly specific response without changing the overall protein levels within the biofilm cells or the overall levels of the extracellular matrix components. The presented results emphasize the central role of the Gram-positive cell wall in biofilm development, resistance and sustainment.

Original languageAmerican English
Pages (from-to)990-1004
Number of pages15
JournalEnvironmental Microbiology Reports
Volume7
Issue number6
DOIs
StatePublished - 1 Dec 2015
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.

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