TY - JOUR
T1 - DksA regulates RNA polymerase in Escherichia coli through a network of interactions in the secondary channel that includes Sequence Insertion 1
AU - Parshin, Andrey
AU - Shiver, Anthony L.
AU - Lee, Jookyung
AU - Ozerova, Maria
AU - Schneidman-Duhovny, Dina
AU - Gross, Carol A.
AU - Borukhov, Sergei
PY - 2015/12/15
Y1 - 2015/12/15
N2 - Sensing and responding to nutritional status is a major challenge for microbial life. In Escherichia coli, the global response to amino acid starvation is orchestrated by guanosine-3′,5′-bisdiphosphate and the transcription factor DksA. DksA alters transcription by binding to RNA polymerase and allosterically modulating its activity. Using genetic analysis, photo-cross-linking, and structural modeling, we show that DksA binds and acts upon RNA polymerase through prominent features of both the nucleotide-access secondary channel and the active-site region. This work is, to our knowledge, the first demonstration of a molecular function for Sequence Insertion 1 in the β subunit of RNA polymerase and significantly advances our understanding of how DksA binds to RNA polymerase and alters transcription.
AB - Sensing and responding to nutritional status is a major challenge for microbial life. In Escherichia coli, the global response to amino acid starvation is orchestrated by guanosine-3′,5′-bisdiphosphate and the transcription factor DksA. DksA alters transcription by binding to RNA polymerase and allosterically modulating its activity. Using genetic analysis, photo-cross-linking, and structural modeling, we show that DksA binds and acts upon RNA polymerase through prominent features of both the nucleotide-access secondary channel and the active-site region. This work is, to our knowledge, the first demonstration of a molecular function for Sequence Insertion 1 in the β subunit of RNA polymerase and significantly advances our understanding of how DksA binds to RNA polymerase and alters transcription.
KW - Lineage-specific insertions
KW - Molecular modeling
KW - Protein cross-linking
KW - Stringent response
KW - Transcription regulation
UR - http://www.scopus.com/inward/record.url?scp=84950105749&partnerID=8YFLogxK
U2 - 10.1073/pnas.1521365112
DO - 10.1073/pnas.1521365112
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 26604313
AN - SCOPUS:84950105749
SN - 0027-8424
VL - 112
SP - E6862-E6871
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 50
ER -