DNA fingerprint analysis of ornamental plants

Galil Tzuri, Jossi Hillel, Uri Lavi, Alon Haberfeld, Alexander Vainstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Minisatellite and microsatellite DNA probes hybridize to many loci in rose, gerbera and carnation DNA, allowing cultivar identification in these ornamentals. The level of polymorphism revealed by DNA fingerprints is probe/genus dependent. Jeffrey's probe 33.6 yielded DNA fingerprints in all genera analyzed, while Jeffreys' probe 33.15 did not yield any detectable signal in gerbera. Cattle derived probe R18.1 detected a higher proportion of polymorphic DNA fingerprint bands in carnation than did any of the other probes. Hybridization of the oligonucleotide probe (GT)12 revealed that simple GT microsatellite repeats generated the hybridization pattern obtained by probe R18.1. Full-sib family analysis of DNA fingerprints prepared from 'standard' Dianthus caryophyllus progeny revealed 36 scorable parental specific bands out of the 186 observed in the parents. These bands were detected cumulatively by phage (M13), cattle (R18.1) and human derived (33.6, 33.15) probes. Only two pairs of these loci were found to be linked. No allelic pairs were detected in the analyzed progeny. The probability of two offspring from a 'standard' carnation intraspecific cross having identical DNA fingerprint patterns was calculated as 1.8 × 10-6.

Original languageAmerican English
Pages (from-to)91-97
Number of pages7
JournalPlant Science
Volume76
Issue number1
DOIs
StatePublished - 1991

Bibliographical note

Funding Information:
The authors wish to thank A.J. Jeffreys for the use of probes 33.6 and 33.15. We also thank Dr. N. Zieslin, Dr. Y. Shoub and R. Shemi Ltd. for providing us with plant materials. This work was supported by a grant from the Shonbrun foundation to A. V. Development of the R 18.1 probe used in this study was partly supported by a grant from the National Council for Research and Development to J.H.

Keywords

  • DNA fingerprints
  • carnation
  • microsatellites
  • minisatellites
  • ornamentals

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