Abstract
DNA probes specific for Mycoplasma pneumoniae and Mycoplasma genitalium were selected from genomic libraries prepared in pUC13. The 32P-labeled probes could detect, by dot blot hybridizaqtion, down to about 0.1 ng of the specific mycoplasma DNA or 105 CFU. Biotinylation of probe decreased the sensitivity of detection and produced nonspecific background reactions with nonhomologous DNAs. Sulfonation of probe yielded a similar level of sensitivity with less background.
Original language | English |
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Pages (from-to) | 726-728 |
Number of pages | 3 |
Journal | Journal of Clinical Microbiology |
Volume | 25 |
Issue number | 4 |
DOIs | |
State | Published - 1987 |