DNA probes for detection and identification of Mycoplasma pneumoniae and Mycoplasma genitalium

H. C. Hyman, D. Yogev, S. Razin

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

DNA probes specific for Mycoplasma pneumoniae and Mycoplasma genitalium were selected from genomic libraries prepared in pUC13. The 32P-labeled probes could detect, by dot blot hybridizaqtion, down to about 0.1 ng of the specific mycoplasma DNA or 105 CFU. Biotinylation of probe decreased the sensitivity of detection and produced nonspecific background reactions with nonhomologous DNAs. Sulfonation of probe yielded a similar level of sensitivity with less background.

Original languageEnglish
Pages (from-to)726-728
Number of pages3
JournalJournal of Clinical Microbiology
Volume25
Issue number4
DOIs
StatePublished - 1987

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