DNA probes for detection and identification of Mycoplasma pneumoniae and Mycoplasma genitalium

H. C. Hyman; D. Yogev; S. Razin

doi:10.1128/jcm.25.4.726-728.1987

DNA probes for detection and identification of Mycoplasma pneumoniae and Mycoplasma genitalium

H. C. Hyman
, D. Yogev
, S. Razin

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

DNA probes specific for Mycoplasma pneumoniae and Mycoplasma genitalium were selected from genomic libraries prepared in pUC13. The ³²P-labeled probes could detect, by dot blot hybridizaqtion, down to about 0.1 ng of the specific mycoplasma DNA or 10⁵ CFU. Biotinylation of probe decreased the sensitivity of detection and produced nonspecific background reactions with nonhomologous DNAs. Sulfonation of probe yielded a similar level of sensitivity with less background.

Original language	English
Pages (from-to)	726-728
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	25
Issue number	4
DOIs	https://doi.org/10.1128/jcm.25.4.726-728.1987
State	Published - 1987

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10.1128/jcm.25.4.726-728.1987

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title = "DNA probes for detection and identification of Mycoplasma pneumoniae and Mycoplasma genitalium",

abstract = "DNA probes specific for Mycoplasma pneumoniae and Mycoplasma genitalium were selected from genomic libraries prepared in pUC13. The 32P-labeled probes could detect, by dot blot hybridizaqtion, down to about 0.1 ng of the specific mycoplasma DNA or 105 CFU. Biotinylation of probe decreased the sensitivity of detection and produced nonspecific background reactions with nonhomologous DNAs. Sulfonation of probe yielded a similar level of sensitivity with less background.",

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AB - DNA probes specific for Mycoplasma pneumoniae and Mycoplasma genitalium were selected from genomic libraries prepared in pUC13. The 32P-labeled probes could detect, by dot blot hybridizaqtion, down to about 0.1 ng of the specific mycoplasma DNA or 105 CFU. Biotinylation of probe decreased the sensitivity of detection and produced nonspecific background reactions with nonhomologous DNAs. Sulfonation of probe yielded a similar level of sensitivity with less background.

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ER -

DNA probes for detection and identification of Mycoplasma pneumoniae and Mycoplasma genitalium

Abstract

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