TY - JOUR
T1 - DNA-repair characterization of cdc40-1, a cell-cycle mutant of Saccharomyces cerevisiae
AU - Kupiec, Martin
AU - Simchen, Giora
PY - 1986/8
Y1 - 1986/8
N2 - The cell-cycle specific mutation cdc-1, which has been previously shown to be sensitive to MMS at the restrictive temperature, was further characterized as a DNA-repair-deficient mutation. cdc40-1 mutants shown only slight to UV irradiation. Double mutant studies shown that rad6-1 is epistatic to cdc40-1 with respect to sensitivity to UV irradiation and MMS. rad50-1 is epistatic to cdc40-1 with respect to MMS sensitivity in G1 stationary cells, but not in logarithmic cultures. An additive effect is seen between cdc40-1 and rad50-1 with respect to UV irradiation. cdc40-1 mutants are defective in UV-induced mutagenesis at the restrictive temperature. UV-induced levels of recombinant are normal at both temperatures, while MMS-induced recombination is enhanced at the restrictive temperature.
AB - The cell-cycle specific mutation cdc-1, which has been previously shown to be sensitive to MMS at the restrictive temperature, was further characterized as a DNA-repair-deficient mutation. cdc40-1 mutants shown only slight to UV irradiation. Double mutant studies shown that rad6-1 is epistatic to cdc40-1 with respect to sensitivity to UV irradiation and MMS. rad50-1 is epistatic to cdc40-1 with respect to MMS sensitivity in G1 stationary cells, but not in logarithmic cultures. An additive effect is seen between cdc40-1 and rad50-1 with respect to UV irradiation. cdc40-1 mutants are defective in UV-induced mutagenesis at the restrictive temperature. UV-induced levels of recombinant are normal at both temperatures, while MMS-induced recombination is enhanced at the restrictive temperature.
UR - http://www.scopus.com/inward/record.url?scp=0022536423&partnerID=8YFLogxK
U2 - 10.1016/0027-5107(86)90068-0
DO - 10.1016/0027-5107(86)90068-0
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C2 - 3523226
AN - SCOPUS:0022536423
SN - 0027-5107
VL - 162
SP - 33
EP - 40
JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
IS - 1
ER -